Letsch Anne, Scheibenbogen Carmen
University Hospital Benjamin-Franklin, Medizinische Klinik III, Hematology, Oncology and Transfusion Medicine, Hindenburgdamm 30, Free University of Berlin, Berlin D-12200, Germany.
Methods. 2003 Oct;31(2):143-9. doi: 10.1016/s1046-2023(03)00124-5.
The novel generation of sensitive T-cell assays facilitates the direct quantitation and characterization of specific T-cell responses. Functional T-cell assays such as the ELISPOT assay and the intracellular cytokine cytometry (ICC) employ the antigen-specific induction of cytokines to detect specific T-cells on a single cell level. ICC has the advantage that the simultaneous phenotypic characterization of the antigen-specific T-cells is possible. There is evidence now from clinical cancer vaccination trials, that there is a relationship between the detection of vaccine-induced T-cells by cytokine-based assays and clinical responses. As these assays become increasingly relevant in clinical practice to suggest issues of assay validation and quality control become of major importance.
新一代灵敏的T细胞检测方法有助于直接定量和表征特异性T细胞反应。功能性T细胞检测方法，如酶联免疫斑点（ELISPOT）检测法和细胞内细胞因子流式细胞术（ICC），利用细胞因子的抗原特异性诱导在单细胞水平上检测特异性T细胞。ICC的优势在于能够同时对抗抗原特异性T细胞进行表型表征。目前临床癌症疫苗试验的证据表明，基于细胞因子的检测法检测到的疫苗诱导T细胞与临床反应之间存在关联。随着这些检测方法在临床实践中变得越来越重要，检测方法的验证和质量控制问题也变得至关重要。
