巴西固氮螺菌谷氨酰胺合成酶编码基因glnA中不同点突变导致的表型变化。
Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene, encoding glutamine synthetase.
作者信息
Van Dommelen Anne, Keijers Veerle, Wollebrants An, Vanderleyden Jozef
机构信息
Center of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Heverlee, Belgium.
出版信息
Appl Environ Microbiol. 2003 Sep;69(9):5699-701. doi: 10.1128/AEM.69.9.5699-5701.2003.
Abstract
Sequencing the glnA genes of two chemically induced Azospirillum brasilense glutamine synthetase mutants revealed an Arg-->Cys mutation, corresponding to the glutamate binding site, in one mutant and an Asp-->Asn mutation, corresponding to the ammonium binding site, in the second mutant. The phenotypic changes in these mutants are discussed in relation to their genotypes.
摘要
对两个化学诱导的巴西固氮螺菌谷氨酰胺合成酶突变体的glnA基因进行测序，结果显示，在一个突变体中，对应于谷氨酸结合位点的发生了Arg→Cys突变，在第二个突变体中，对应于铵结合位点的发生了Asp→Asn突变。本文结合这些突变体的基因型对其表型变化进行了讨论。
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