Tanaka Reiko, Imanishi Yumi, Nishimura Kazuko
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan.
J Clin Microbiol. 2003 Sep;41(9):4457-9. doi: 10.1128/JCM.41.9.4457-4459.2003.
We compared sequences of the glucan synthase (FKS1) gene in serotypes A and D of Cryptococcus neoformans. Four introns were present in serotype D but not serotype A. PCR with primers that flank these introns permits simple differentiation of serotypes A and D.
我们比较了新型隐球菌A和D血清型中葡聚糖合酶(FKS1)基因的序列。血清型D中有四个内含子,而血清型A中没有。使用位于这些内含子两侧的引物进行PCR可简单区分血清型A和D。