通过16S rRNA基因的PCR-限制性片段长度多态性分析鉴定口腔消化链球菌分离株。
Identification of oral peptostreptococcus isolates by PCR-restriction fragment length polymorphism analysis of 16S rRNA genes.
作者信息
Riggio Marcello P, Lennon Alan
机构信息
Infection and Immunity Research Group, University of Glasgow Dental School, Glasgow G2 3JZ, United Kingdom.
出版信息
J Clin Microbiol. 2003 Sep;41(9):4475-9. doi: 10.1128/JCM.41.9.4475-4479.2003.
Abstract
Oral Peptostreptococcus isolates tentatively identified by conventional microbiological culture methods were identified to the species level by a combination of PCR amplification of 16S rRNA genes and restriction enzyme analysis of the amplified products. This method is a reliable and rapid alternative to conventional methods for identification of these bacterial species.
摘要
通过常规微生物培养方法初步鉴定的口腔消化链球菌分离株，采用16S rRNA基因的PCR扩增和扩增产物的限制性酶切分析相结合的方法进行种水平鉴定。该方法是用于鉴定这些细菌种类的传统方法的可靠且快速的替代方法。
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