Ng Y K, Wong W C, Ling E A
Department of Anatomy, Faculty of Medicine, National University of Singapore.
J Anat. 1992 Oct;181 ( Pt 2)(Pt 2):249-58.
A morphometric study was undertaken on the submandibular ganglion cells in rats of different ages. This showed a direct proportional increase with age in all the variables measured. Mean cross-sectional cell area showed the most dramatic growth, an increase of more than 5-fold between birth and young adulthood. Mean cell diameter and cell perimeter doubled over the same period. The growth of the nucleus, expressed as diameter, was slower when compared with that of the ganglion cells as a whole. The number of intraglandular ganglion cells remained relatively unchanged from birth to young adulthood, ranging from about 3000 to 5000 cells. They were mainly distributed at the hilar region of the submandibular salivary gland, contributing 1/2 to 2/3 of the total ganglion cell population. The second largest cell population was in the intralobular region, which made up about one-third of the population. The least populated region was in the connective tissue of the sublingual salivary gland, which contained only about 5-7% of the total cell number. Cell counts based on the fluorogold labelling method were generally lower than those made after haematoxylin and eosin staining. In the 2-d-old animals, counts of fluorogold-labelled cells were only about half the H & E counts. The discrepancy may be due to the thicker sections used in the fluorogold method, superimposition of cells leading to an underestimation of cell numbers. Nevertheless, the fluorogold labelling method provided rapid and reproducible results. Its main advantage is that the labelled ganglion cells emit a bright yellow fluorescence which is readily identified; the other is the simplicity of the procedure, as labelling of ganglion cells can be achieved by the intraperitoneal route.
对不同年龄大鼠的下颌下神经节细胞进行了形态计量学研究。结果显示，所测量的所有变量均随年龄呈正比增加。平均细胞横截面积增长最为显著，从出生到青年期增加了5倍多。同期平均细胞直径和细胞周长增加了一倍。以直径表示的细胞核生长速度比整个神经节细胞的生长速度慢。腺内神经节细胞的数量从出生到青年期相对保持不变，约为3000至5000个细胞。它们主要分布在下颌下唾液腺的门部区域，占神经节细胞总数的1/2至2/3。第二大细胞群位于小叶内区域，约占总数的三分之一。细胞数量最少的区域是舌下唾液腺的结缔组织，仅占细胞总数的约5 - 7%。基于荧光金标记法的细胞计数通常低于苏木精和伊红染色后的计数。在2日龄动物中，荧光金标记细胞的计数仅约为苏木精和伊红染色计数的一半。这种差异可能是由于荧光金法使用的切片较厚，细胞重叠导致细胞数量估计偏低。然而，荧光金标记法提供了快速且可重复的结果。其主要优点是标记的神经节细胞发出明亮的黄色荧光，易于识别；另一个优点是操作简单，因为通过腹腔途径即可实现神经节细胞的标记。
