Vesely P, Boyde A, Jones S J
Department of Anatomy and Developmental Biology, University College London, UK.
J Anat. 1992 Oct;181 ( Pt 2)(Pt 2):277-91.
The cell-cell contact-induced behaviour of osteoclasts and osteoblasts in vitro was investigated by time-lapse videomicroscopy. Contact interactions of osteoclasts with autologous cells, derived mostly from chick but also from rat bones, included contact inhibition, failure of contact inhibition, contact guidance along stabilised edges of other cells, and underlapping of other cells. Message-mediated contact behaviour (MMCB) between osteoclasts and autologous osteoblastic cells resulted, after a time delay, in zeiosis of the osteoblast-like cell which could continue, or even begin, after the osteoclast broke contact, leading to retraction of the cell and occupation of its position by the osteoclast. MMCB may play a part in the breaching of the osteoblastic sheet by osteoclasts and, in general, in the malignant spread of neoplastic cells. Two or more osteoclasts were often joined by connecting and coordinating tubules (CCTs) of varied, and varying, lengths and widths. Osteoclasts could travel along the CCTs in both directions, or send nuclei through them. The CCTs became temporarily attached to the surface of other cells, or to the substrate, then acting as a temporary anchorage for orientation and for the return of the cell to the same spot. The dynamics of osteoclastic behaviour suggest that such a networking of osteoclasts is valuable for the 3D coordination of their role in bone turnover.
通过延时视频显微镜研究了破骨细胞和成骨细胞在体外细胞间接触诱导的行为。破骨细胞与主要来源于鸡骨但也有大鼠骨的自体细胞的接触相互作用包括接触抑制、接触抑制失败、沿其他细胞稳定边缘的接触导向以及其他细胞的重叠。破骨细胞与自体成骨细胞之间的信息介导接触行为（MMCB）在一段时间延迟后，导致成骨样细胞出现沸腾现象，这种现象在破骨细胞断开接触后仍可继续，甚至开始，导致细胞回缩，破骨细胞占据其位置。MMCB可能在破骨细胞突破成骨细胞层中起作用，并且一般在肿瘤细胞的恶性扩散中起作用。两个或更多破骨细胞常通过长度和宽度各异且不断变化的连接和协调小管（CCT）连接在一起。破骨细胞可以沿CCT双向移动，或者将细胞核送过它们。CCT会暂时附着在其他细胞表面或底物上，然后作为细胞定向和回到同一点的临时锚定物。破骨细胞行为的动态变化表明，这种破骨细胞网络对于其在骨转换中的作用的三维协调是有价值的。
