Veselý P, Chaloupková A, Urbanec P, Urbancová H, Bohác L, Krchnáková E, Franc F, Sprincl L, Vousden K, Moss R
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Folia Biol (Praha). 1987;33(5):307-24.
Cells of K2M rat sarcoma and their descendants (RP, RPS) form tumours in syngeneic inbred rat strain LEW/CUB. Thirty to fifty per cent of tumour-bearing rats develop metastases, preferentially in the lung. In vitro, a quasi-stellate dynamic morphotype was characteristic of K2M cells and their descendants (RP, RPS): a highly changeable round or spindle-shaped or multipolar rather thick cell body with two or more protrusions of various length, often branching, and broadened at the tips, where vivid membrane activity was observed. Changeability of appearance and directionality of locomotion were stimulated in medium of pH 6.5. The enhanced expression of this morphotype correlated with the increased metastatic potential. In vitro, in poor, slightly acid medium metastasizing cells preserved the quasi-stellate dynamic morphotype and survived longer than non-metastasizing cells. It is likely that the observed combination of traits, viz. the quasi-stellate dynamic morphotype and, under unfavourable conditions, prolonged survival, increased migratory activity and decreased mitotic rate could be of significance in the metastatic cascade, mainly for the escape from the primary site.
K2M大鼠肉瘤细胞及其子代细胞（RP、RPS）在同基因近交系大鼠LEW/CUB体内形成肿瘤。30%至50%的荷瘤大鼠会发生转移，且优先转移至肺部。在体外，K2M细胞及其子代细胞（RP、RPS）具有一种准星状动态形态类型：细胞体高度可变，呈圆形、纺锤形或多极形，相当厚实，有两个或更多长短不一的突起，常呈分支状，末端变宽，在末端可观察到活跃的膜活动。在pH 6.5的培养基中，细胞外观的可变性和运动的方向性会受到刺激。这种形态类型的增强表达与转移潜能的增加相关。在体外，在贫瘠、微酸性的培养基中，具有转移能力的细胞保持准星状动态形态类型，并且比不具有转移能力的细胞存活时间更长。所观察到的特征组合，即准星状动态形态类型，以及在不利条件下延长的存活时间、增加的迁移活性和降低的有丝分裂率，可能在转移级联反应中具有重要意义，主要是对于从原发部位逃脱而言。
