[银溶胶中AMP和DNA的表面增强拉曼散射（SERS）光谱]

[Surface enhanced Raman scattering (SERS) spectra of AMP and DNA in silver sol].

作者信息

Shen H B, Xia J F, Zhang F, Yang H F, Zhang Z R

机构信息

Shanghai Teachers University, Department of Chemistry, Shanghai 200234, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Dec;21(6):798-800.

PMID:12958898

Abstract

Surface enhanced Raman scattering (SERS) spectra of AMP and DNA in silver sol were obtained. Comparing the SERS spectra with spectra of AMP and DNA in solid state, we found that although those two systems were very similar in the main, but there were still some differences between them. For example, the ring breathing modes of adenine in solid AMP at 715 cm-1 was enhanced very much and appears at 723 cm-1 in silver sol. The symmetric stretching mode of phosphate group at 813 cm-1 was disappeared in SERS spectra. The vibration mode of ribose in DNA was enhanced obviously in SERS spectra, four kinds bands of A, T, C, G were also enhanced in varying degrees. The possible adsorption model of AMP and DNA in silver sol and the structure change is proposed.

摘要

获得了银溶胶中AMP和DNA的表面增强拉曼散射（SERS）光谱。将SERS光谱与固态AMP和DNA的光谱进行比较，我们发现虽然这两个体系总体上非常相似，但它们之间仍存在一些差异。例如，固态AMP中腺嘌呤在715 cm-1处的环呼吸模式在银溶胶中增强很多并出现在723 cm-1处。813 cm-1处磷酸基团的对称拉伸模式在SERS光谱中消失。DNA中核糖的振动模式在SERS光谱中明显增强，A、T、C、G的四条谱带也有不同程度的增强。提出了AMP和DNA在银溶胶中的可能吸附模型及结构变化。