Zhao X, Zhang H
Hebei Medical College Education, 071000 Baoding.
Guang Pu Xue Yu Guang Pu Fen Xi. 2000 Jun;20(3):431-3.
In this paper an isochromatic dye ion-pairs extraction-catalytic spectrophotometric method for the determination of trace copper is studied. The method is based on the copper catalyzed oxidation of isochromatic dye ion-pairs formed by buthylrhodamine B and bromophenol blue by hydrogen peroxide in ammoniac medium. The reaction time, concentration of isochromatic dye ion-pairs in aqueous phase and degree of reaction are controlled by extraction equilibrium. The detection limit of this method is 0.02 microgram.L-1. The linear range is 0.1-10 micrograms.L-1. The method has been applied to the determination of trace copper in tumor tissue with satisfactory results.
本文研究了一种用于测定痕量铜的等色染料离子对萃取-催化分光光度法。该方法基于在氨介质中，铜催化过氧化氢氧化丁基罗丹明B与溴酚蓝形成的等色染料离子对。通过萃取平衡控制反应时间、水相中染料离子对的浓度和反应程度。该方法的检出限为0.02μg·L-1，线性范围为0.1~10μg·L-1。该方法已用于肿瘤组织中痕量铜的测定，结果令人满意。
