[Extraction-catalytic kinetic spectrophotometric determination of trace element in tumor tissue].

In this paper an isochromatic dye ion-pairs extraction-catalytic spectrophotometric method for the determination of trace copper is studied. The method is based on the copper catalyzed oxidation of isochromatic dye ion-pairs formed by buthylrhodamine B and bromophenol blue by hydrogen peroxide in ammoniac medium. The reaction time, concentration of isochromatic dye ion-pairs in aqueous phase and degree of reaction are controlled by extraction equilibrium. The detection limit of this method is 0.02 microgram.L-1. The linear range is 0.1-10 micrograms.L-1. The method has been applied to the determination of trace copper in tumor tissue with satisfactory results.