Maher P A
Department of Molecular and Cellular Growth Biology, Whittier Institute for Diabetes and Endocrinology, La Jolla, California 92037.
J Cell Physiol. 1992 Jun;151(3):549-54. doi: 10.1002/jcp.1041510314.
Micromolar concentrations of sodium orthovanadate stimulated the proliferation of bovine capillary endothelial cells, but not bovine aortic endothelial cells. Vanadate was equally potent at inducing protein tyrosine phosphorylation and changes in morphology in both types of cells. However, vanadate treatment lead to an inhibition of protein tyrosine kinase activity in the aortic endothelial cells, but not the capillary endothelial cells. In capillary endothelial cells, the effect of vanadate was additive with basic FGF (bFGF) at low concentrations of bFGF. There was no interaction between bFGF and vanadate in aortic endothelial cells. TGF-beta, which inhibits the induction of endothelial cell proliferation by bFGF, appeared to shift the dose response curve to vanadate in capillary endothelial cells, increasing the proliferative effect of vanadate at low vanadate concentrations, but decreasing the proliferative effect at higher vanadate concentrations.
微摩尔浓度的原钒酸钠能刺激牛毛细血管内皮细胞的增殖，但对牛主动脉内皮细胞无此作用。钒酸盐在诱导两种细胞的蛋白质酪氨酸磷酸化和形态变化方面具有同等效力。然而，钒酸盐处理导致主动脉内皮细胞中的蛋白质酪氨酸激酶活性受到抑制，而毛细血管内皮细胞则不受影响。在毛细血管内皮细胞中，在低浓度碱性成纤维细胞生长因子（bFGF）的情况下，钒酸盐的作用与bFGF具有相加性。在主动脉内皮细胞中，bFGF与钒酸盐之间没有相互作用。转化生长因子-β（TGF-β）可抑制bFGF诱导的内皮细胞增殖，它似乎使毛细血管内皮细胞对钒酸盐的剂量反应曲线发生偏移，在低钒酸盐浓度时增加了钒酸盐的增殖作用，但在高钒酸盐浓度时降低了增殖作用。
