Brück Wolfram M, Kelleher Shannon L, Gibson Glenn R, Nielsen Karin E, Chatterton Dereck E W, Lönnerdal Bo
Food Microbial Sciences Unit, School of Food Biosciences, The University of Reading, Reading, RG6 6AP, UK.
J Pediatr Gastroenterol Nutr. 2003 Sep;37(3):273-80. doi: 10.1097/00005176-200309000-00014.
Certain milk factors may help to promote the growth of a host-friendly colonic microflora (e.g. bifidobacteria, lactobacilli) and explain why breast-fed infants experience fewer and milder intestinal infections than those who are formula-fed. The effects of supplementation of formula with two such milk factors was investigated in this study.
Infant rhesus macaques were breast-fed, fed control formula, or formula supplemented with glycomacropeptide (GMP) or alpha-lactalbumin (alpha-LA) from birth to 5 months of age. Blood was drawn monthly and rectal swabs were collected weekly. At 4.5 months of age, 10(8) colony-forming units of enteropathogenic E.coli O127, strain 2349/68 (EPEC) was given orally and the response to infection assessed. The bacteriology of rectal swabs pre- and post-infection was determined by culture independent fluorescence in situ hybridization.
Post-challenge, breast-fed infants and infants fed alpha-LA-supplemented formula had no diarrhea, whilst those infants fed GMP-supplemented formula had intermittent diarrhea. In infants fed control formula the diarrhea was acute.
Supplementation of infant formula with appropriate milk proteins may be useful for improving the infant's ability to resist acute infection caused by E.coli.
某些牛奶成分可能有助于促进对宿主有益的结肠微生物群（如双歧杆菌、乳酸杆菌）的生长，并解释为什么母乳喂养的婴儿比配方奶喂养的婴儿患肠道感染的次数更少且症状更轻。本研究调查了在配方奶中添加两种此类牛奶成分的效果。
从出生到5个月大，对恒河猴幼崽进行母乳喂养、喂对照配方奶或添加了糖巨肽（GMP）或α-乳白蛋白（α-LA）的配方奶。每月采集血液，每周收集直肠拭子。在4.5个月大时，口服10⁸ 菌落形成单位的肠致病性大肠杆菌O127菌株2349/68（EPEC），并评估感染反应。通过非培养荧光原位杂交法确定感染前后直肠拭子的细菌学情况。
在受到挑战后，母乳喂养的婴儿和喂食添加α-LA配方奶的婴儿没有腹泻，而喂食添加GMP配方奶的婴儿有间歇性腹泻。喂食对照配方奶的婴儿腹泻是急性的。
在婴儿配方奶中添加适当的乳蛋白可能有助于提高婴儿抵抗大肠杆菌引起的急性感染的能力。
