García-Jiménez Angela, Fastbom Johan, Ohm Thomas G, Cowburn Richard F
Neurotec Department, Division of Experimental Geriatrics, Karolinska Institutet, Novum, KFC, S-141 86 Huddinge, Sweden.
Neuroreport. 2003 Aug 6;14(11):1523-7. doi: 10.1097/00001756-200308060-00025.
G-protein alpha-subunits (Galphao, Galphai, Galphas, Galphaq) and adenylyl cyclase (AC) I and II isoforms were quantified in hippocampus and entorhinal cortex from 22 cases staged for Alzheimer's disease (AD) pathologies according to Braak and Braak. Hippocampal Galphai levels declined significantly with neurofibrillary staging, whereas AC I levels in this region increased. Significant amyloid stage-related reductions of Galphai were seen in both the hippocampus and entorhinal cortex. The hippocampus also showed a significant reduction of Galphao with amyloid staging. It is concluded that levels of inhibitory G-protein subunits Galphao, and in particular Galphai, decrease in parallel to the extent of AD pathology.
根据Braak和Braak分期标准，对22例阿尔茨海默病（AD）病理分期患者的海马体和内嗅皮质中的G蛋白α亚基（Galphao、Galphai、Galphas、Galphaq）以及腺苷酸环化酶（AC）I和II亚型进行了定量分析。海马体中Galphai水平随神经纤维缠结分期显著下降，而该区域的AC I水平则升高。在海马体和内嗅皮质中均观察到与淀粉样蛋白阶段相关的Galphai显著减少。随着淀粉样蛋白分期，海马体中的Galphao也显著减少。研究得出结论，抑制性G蛋白亚基Galphao，尤其是Galphai的水平与AD病理程度呈平行下降。
