氢化物发生原子荧光光谱法测定中草药中的硒

[Determination of selenium in Chinese herbs by hydride generation atomic fluorescence spectrometry].

作者信息

Yang Li-li, Zhang De-qiang, Gao Ying, Yuan Chun-gang, Zhang Yan-xin, Sun Han-wen

机构信息

College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2003 Apr;23(2):368-70.

PMID:12961898

Abstract

A method for the determination of selenium by hydride generation atomic fluorescence spectrometry (HG-AFS) was proposed in this paper. The experimental conditions including the temperature of atomizer, height of atomizer, lamp current, flow rate of carrier gas, concentration of KBH4, and acidic medium that affect the generation of hydride and the sensitivity of the method were investigated and optimized. The influence of coexisting foreign ions on the determination of selenium was also investigated. The detection limit of the proposed method was found to be 0.21 ng.mL-1. The proposed method was successfully applied to the determination of selenium in ten kinds of Chinese herbs, and the recoveries were in the range of 95%-105%.

摘要

本文提出了一种用氢化物发生原子荧光光谱法（HG-AFS）测定硒的方法。研究并优化了包括原子化器温度、原子化器高度、灯电流、载气流量、KBH₄浓度以及影响氢化物生成和方法灵敏度的酸性介质等实验条件。还研究了共存外来离子对硒测定的影响。该方法的检出限为0.21 ng·mL⁻¹。该方法成功应用于十种中草药中硒的测定，回收率在95% - 105%范围内。