[采用氢化物发生原子荧光光谱法对中药中的汞进行形态分析]

[Speciation analysis of mercury in traditional Chinese medicine by vapor-generation atomic fluorescence spectrometry].

作者信息

Yang Li-li, Li Na, Zhang De-qiang, Gao Li-rong, Kang Hai-yan

机构信息

College of Chemistry, Hebei Normal University, Shijiazhuang 050016, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Feb;25(2):286-9.

PMID:15852879

Abstract

A sensitive method has been proposed for the determination of inorganic mercury and total organic mercury in traditional Chinese medicine (Wanshi Niuhuang Qingxin) by vapor-generation atomic fluorescence spectrometry. The experimental conditions that influence the atomic fluorescence signal intensity of Hg and the oxidization of organic mercury were investigated and optimized. Thiourea citric acid was selected as a sensitization agent, which greatly enhanced atomic fluorescence signal intensity of mercury. The influence of foreign ions and their elimination were studied. The detection limit of the method for Hg was 7.6 ng x L(-1) and the relative standard deviation was 1.56%-3.28% for Hg. The proposed method was successfully applied to the determination of mercury speciation in real samples with a recovery range of 90.3%-110.3%.

摘要

提出了一种采用蒸气发生原子荧光光谱法测定中药（万氏牛黄清心）中无机汞和总有机汞的灵敏方法。研究并优化了影响汞原子荧光信号强度及有机汞氧化的实验条件。选用硫脲柠檬酸作为增敏剂，极大地增强了汞的原子荧光信号强度。研究了外来离子的影响及其消除方法。该方法对汞的检出限为7.6 ng·L⁻¹，汞的相对标准偏差为1.56% - 3.28%。所提方法成功应用于实际样品中汞形态的测定，回收率范围为90.3% - 110.3%。