Ogata M, Fujii Y
Department of Public Health, Okayama University Medical School, Japan.
Physiol Chem Phys Med NMR. 1992;24(4):261-9.
Catalase in hemolysates of normal, heterozygous hypocatalasemic and acatalasemic Japanese was immunotitrated with an anti-human blood catalase rabbit serum. Equivalence points were calculated from the regression lines between catalase activity added and catalase activity remaining in the supernatant. Catalase activities at the equivalence points of Japanese normal, hypocatalasemia and acatalasemia were similar. The results indicate that the specific activities of catalase in the normal and of the variant bloods are identical. Catalase in hemolysates of normal and variant mice was immunotitrated with an anti-mouse liver catalase rabbit serum. In contrast to Japanese acatalasemic subject, the equivalence points of catalase in heterozygous hypocatalasemic, homozygous hypocatalasemic, acatalasemic and normal hemolysates were different, and the ratios of specific activity in these variant mice to that in normal were 0.72, 0.46 and 0.21, respectively. The differences in catalase activities at equivalence points were also supported by the statistical analysis on parameters of regression lines of catalase activities remaining in the supernatant on catalase activities added in the immunotitration. These findings suggest that the molecular properties of residual catalase of Japanese acatalasemia and those of mouse acatalasemia are entirely different.
用抗人血过氧化氢酶兔血清对正常、杂合子低过氧化氢酶血症和无过氧化氢酶血症的日本人的溶血产物中的过氧化氢酶进行免疫滴定。根据加入的过氧化氢酶活性与上清液中剩余的过氧化氢酶活性之间的回归线计算等价点。日本人正常、低过氧化氢酶血症和无过氧化氢酶血症等价点处的过氧化氢酶活性相似。结果表明，正常血液和变异血液中过氧化氢酶的比活性相同。用抗小鼠肝脏过氧化氢酶兔血清对正常和变异小鼠的溶血产物中的过氧化氢酶进行免疫滴定。与日本人无过氧化氢酶血症患者不同，杂合子低过氧化氢酶血症、纯合子低过氧化氢酶血症、无过氧化氢酶血症和正常溶血产物中过氧化氢酶的等价点不同，这些变异小鼠与正常小鼠的比活性之比分别为0.72、0.46和0.21。免疫滴定中上清液中剩余的过氧化氢酶活性与加入的过氧化氢酶活性的回归线参数的统计分析也支持了等价点处过氧化氢酶活性的差异。这些发现表明，日本人无过氧化氢酶血症残留过氧化氢酶的分子特性与小鼠无过氧化氢酶血症的分子特性完全不同。
