Ogata M, Satoh Y
Department of Public Health, Okayama University Medical School, Japan.
Electrophoresis. 1988 Mar;9(3):128-31. doi: 10.1002/elps.1150090305.
Hemolysates of normal, heterozygous hypocatalasemic and acatalasemic mice and of Japanese acatalasemic subjects were separated into three fractions, A, B and C, by DEAE-cellulose column chromatography, and pI values of A, B and C fractions were determined by isoelectric focusing. The pI value of catalase in the A, B and C fractions increased in the order of normal, hypocatalasemic and acatalasemic mouse blood. The results obtained from Japanese acatalasemic blood samples showed that the pI values of catalase in the A, B and C fractions were similar to those in normal blood. Catalase in Japanese acatalasemic cultured skin fibroblasts was also analyzed by isoelectric focusing. The pI values of catalase in the extract from the cultured skin acatalasemic fibroblasts was similar to that in normal fibroblasts.
通过DEAE - 纤维素柱色谱法将正常、杂合子低过氧化氢酶血症和无过氧化氢酶血症小鼠以及日本无过氧化氢酶血症受试者的溶血产物分离为A、B和C三个组分，并通过等电聚焦法测定A、B和C组分的pI值。A、B和C组分中过氧化氢酶的pI值按正常、低过氧化氢酶血症和无过氧化氢酶血症小鼠血液的顺序升高。从日本无过氧化氢酶血症血样获得的结果表明，A、B和C组分中过氧化氢酶的pI值与正常血液中的相似。还通过等电聚焦法分析了日本无过氧化氢酶血症培养皮肤成纤维细胞中的过氧化氢酶。无过氧化氢酶血症培养皮肤成纤维细胞提取物中过氧化氢酶的pI值与正常成纤维细胞中的相似。
