通过质谱法和埃德曼测序对人细胞质大核糖体亚基蛋白的翻译后修饰进行表征和分析。
Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing.
作者信息
Odintsova Tatyana I, Müller Eva-Christina, Ivanov Anton V, Egorov Tsezi A, Bienert Ralf, Vladimirov Serguei N, Kostka Susanne, Otto Albrecht, Wittmann-Liebold Brigitte, Karpova Galina G
机构信息
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russian Federation.
出版信息
J Protein Chem. 2003 Apr;22(3):249-58. doi: 10.1023/a:1025068419698.
The 60S ribosomal proteins were isolated from ribosomes of human placenta and separated by reversed phase HPLC. The fractions obtained were subjected to trypsin and Glu-C digestion and analyzed by mass fingerprinting (MALDI-TOF), MS/MS (ESI), and Edman sequencing. Forty-six large subunit proteins were found, 22 of which showed masses in accordance with the SwissProt database (June 2002) masses (proteins L6, L7, L9, L13, L15, L17, L18, L21, L22, L24, L26, L27, L30, L32, L34, L35, L36, L37, L37A, L38, L39, L41). Eleven (proteins L7, L10A, L11, L12, L13A, L23, L23A, L27A, L28, L29, and P0) resulted in mass changes that are consistent with N-terminal loss of methionine, acetylation, internal methylation, or hydroxylation. A loss of methionine without acetylation was found for protein L8 and L17. For nine proteins (L3, L4, L5, L7A, L10, L14, L19, L31, and L40), the molecular masses could not be determined. Proteins P1 and protein L3-like were not identified by the methods applied.
从人胎盘核糖体中分离出60S核糖体蛋白，并通过反相高效液相色谱进行分离。对所得馏分进行胰蛋白酶和Glu-C消化，并通过质谱指纹分析（基质辅助激光解吸电离飞行时间质谱）、串联质谱（电喷雾电离）和埃德曼测序进行分析。共发现46种大亚基蛋白，其中22种的质量与瑞士蛋白质数据库（2002年6月）中的质量相符（蛋白质L6、L7、L9、L13、L15、L17、L18、L21、L22、L24、L26、L27、L30、L32、L34、L35、L36、L37、L37A、L38、L39、L41）。11种（蛋白质L7、L10A、L11、L12、L13A、L23、L23A、L27A、L28、L29和P0）出现了与N端甲硫氨酸缺失、乙酰化、内部甲基化或羟基化一致的质量变化。发现蛋白质L8和L17存在无乙酰化的甲硫氨酸缺失。对于9种蛋白质（L3、L4、L5、L7A、L10、L14、L19、L31和L40），无法确定其分子量。所采用的方法未鉴定出蛋白质P1和类L3蛋白。
Zotero 插件