Yoshihara K, Oshima K, Muneta Y, Kikuma R, Yayota C, Hirai T, Satoh N, Matsuura S, Kikyo Y, Satoh M, Kubota C, Inumaru S, Yokomizo Y, Mori Y
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Vet Immunol Immunopathol. 2003 Oct 15;95(3-4):103-11. doi: 10.1016/s0165-2427(03)00103-x.
We established an enzyme-linked immunosorbent assay (ELISA) system for the quantitation of bovine macrophage colony-stimulating factor (M-CSF) and used it to measure the serum M-CSF levels in bovine fetuses and calves. The average serum M-CSF level was 2.7+/-1.5 ng/ml in 39 calves under 100 days old, and 1.8+/-0.8 ng/ml in 15 cattle between 101 and 418 days old. Fetal sera samples (n = 6) prepared from cattle between 150 and 280 days of gestational age had a higher average level of M-CSF (8.8+/-1.4 ng/ml). Alteration in serum M-CSF levels in each individual calf was also measured. The serum levels of M-CSF in calves at 0-1 day after birth ranged from 0.52 to 7.3 ng/ml. During the period 113-125 days after birth, serum levels were around 1.4+/-0.39 ng/ml. Although serum M-CSF levels generally decreased as the age of calves advanced, differences among individuals, especially among newborn calves, were observed.
我们建立了一种用于定量牛巨噬细胞集落刺激因子（M-CSF）的酶联免疫吸附测定（ELISA）系统，并使用该系统测量牛胎儿和犊牛的血清M-CSF水平。39头100日龄以下犊牛的血清M-CSF平均水平为2.7±1.5 ng/ml，15头101至418日龄牛的血清M-CSF平均水平为1.8±0.8 ng/ml。从150至280日龄孕牛制备的胎儿血清样本（n = 6）的M-CSF平均水平较高（8.8±1.4 ng/ml）。还测量了每头犊牛血清M-CSF水平的变化。出生后0至1天犊牛的血清M-CSF水平在0.52至7.3 ng/ml之间。出生后113至125天期间，血清水平约为1.4±0.39 ng/ml。虽然血清M-CSF水平通常随着犊牛年龄的增长而降低，但观察到个体之间存在差异，尤其是新生犊牛之间。
