Takahashi M, Ohmoto Y, Mizuno K, Yamanishi K, Hirai Y, Adachi M
Cellular Technology Institute, Otsuka Pharmaceutical Co., Ltd. Tokushima, Japan.
Biosci Biotechnol Biochem. 1993 Nov;57(11):1838-43. doi: 10.1271/bbb.57.1838.
A sensitive and reliable radioimmunoassay (RIA) for human macrophage colony-stimulating factor (M-CSF) was developed using recombinant human M-CSF (rhM-CSF) as tracer and immunogen. The assay was quantitative over the range of 50 pg/ml and 5.0 ng/ml for M-CSF in human urine and serum, and more sensitive and specific than the murine bone marrow assay. The average level of human M-CSF in urine from normal males (N = 71) and females (N = 46) was 3.94 +/- 1.78 ng/ml (2.85 +/- 1.15 micrograms/g creatinine), and 3.53 +/- 1.70 ng/ml (3.31 +/- 1.12 micrograms/g creatinine), respectively. The serum levels were 1.95 +/- 0.38 ng/ml for males (N = 117), and 1.93 +/- 0.49 ng/ml for females, (N = 16). The results with the urine and sera showed that there was no difference in the M-CSF levels due to age or gender.
采用重组人巨噬细胞集落刺激因子（rhM-CSF）作为示踪剂和免疫原，建立了一种灵敏可靠的人巨噬细胞集落刺激因子（M-CSF）放射免疫分析方法（RIA）。该检测方法对人尿液和血清中M-CSF的定量范围为50 pg/ml至5.0 ng/ml，比小鼠骨髓检测方法更灵敏、特异。正常男性（N = 71）和女性（N = 46）尿液中M-CSF的平均水平分别为3.94 +/- 1.78 ng/ml（2.85 +/- 1.15微克/克肌酐）和3.53 +/- 1.70 ng/ml（3.31 +/- 1.12微克/克肌酐）。男性（N = 117）血清水平为1.95 +/- 0.38 ng/ml，女性（N = 16）血清水平为1.93 +/- 0.49 ng/ml。尿液和血清检测结果表明，M-CSF水平在年龄或性别上没有差异。
