以重组人巨噬细胞集落刺激因子为示踪剂和免疫原建立人巨噬细胞集落刺激因子的放射免疫测定法。

Establishment of radioimmunoassay for human macrophage colony-stimulating factor using recombinant human macrophage colony-stimulating factor as tracer and immunogen.

作者信息

Takahashi M, Ohmoto Y, Mizuno K, Yamanishi K, Hirai Y, Adachi M

机构信息

Cellular Technology Institute, Otsuka Pharmaceutical Co., Ltd. Tokushima, Japan.

出版信息

Biosci Biotechnol Biochem. 1993 Nov;57(11):1838-43. doi: 10.1271/bbb.57.1838.

DOI:10.1271/bbb.57.1838

PMID:7764334

Abstract

A sensitive and reliable radioimmunoassay (RIA) for human macrophage colony-stimulating factor (M-CSF) was developed using recombinant human M-CSF (rhM-CSF) as tracer and immunogen. The assay was quantitative over the range of 50 pg/ml and 5.0 ng/ml for M-CSF in human urine and serum, and more sensitive and specific than the murine bone marrow assay. The average level of human M-CSF in urine from normal males (N = 71) and females (N = 46) was 3.94 +/- 1.78 ng/ml (2.85 +/- 1.15 micrograms/g creatinine), and 3.53 +/- 1.70 ng/ml (3.31 +/- 1.12 micrograms/g creatinine), respectively. The serum levels were 1.95 +/- 0.38 ng/ml for males (N = 117), and 1.93 +/- 0.49 ng/ml for females, (N = 16). The results with the urine and sera showed that there was no difference in the M-CSF levels due to age or gender.

摘要

采用重组人巨噬细胞集落刺激因子（rhM-CSF）作为示踪剂和免疫原，建立了一种灵敏可靠的人巨噬细胞集落刺激因子（M-CSF）放射免疫分析方法（RIA）。该检测方法对人尿液和血清中M-CSF的定量范围为50 pg/ml至5.0 ng/ml，比小鼠骨髓检测方法更灵敏、特异。正常男性（N = 71）和女性（N = 46）尿液中M-CSF的平均水平分别为3.94 +/- 1.78 ng/ml（2.85 +/- 1.15微克/克肌酐）和3.53 +/- 1.70 ng/ml（3.31 +/- 1.12微克/克肌酐）。男性（N = 117）血清水平为1.95 +/- 0.38 ng/ml，女性（N = 16）血清水平为1.93 +/- 0.49 ng/ml。尿液和血清检测结果表明，M-CSF水平在年龄或性别上没有差异。