Satoh Jin, Tsujikawa Tomoyuki, Fujiyama Yoshihide, Banba Tadao
Division of Gastroenterology, Shiga University of Medical Science, Tsukinowa-cho, Seta, Otsu, Shiga 520-2192, Japan.
Int J Mol Med. 2003 Oct;12(4):615-20.
Although parental administration of glutamine promotes intestinal adaptation, it is controversial whether enteral glutamine is effective after small bowel resection. To further evaluate the benefits of enteral supplementation, peptide and amino acid peptide transporter function must be considered. We evaluated the effect of enteral alanyl-glutamine based on the alteration of peptide and amino acid transporter expressions after massive small intestinal resection. Rats underwent 80% proximal intestinal resection. Expression of the glutaminase (GA), amino acid transporter B0 and peptide transporter PepT1 mRNA in the residual intestinal cells was initially examined by Northern blot analysis. Rats with a small bowel received a bolus supplement of glutamine (2.0 g/kg/day) + alanine (1.22 g/kg/day) mixture, alanyl-glutamine (2.972/kg/day) or saline for 3 days from one day before operation. On the 3rd postoperative day (POD) and the 7th POD, residual intestinal tissue was removed, and mucosal parameters were measured. The GA activity and GA mRNA significantly increased on the 1st POD. Although the levels of B0 mRNA gradually decreased, the PepT1 mRNA increased after surgery, and reached 150% of the initial level on the 5th POD. In the rats administered alanyl-glutamine, mucosal wet weight and protein content similarly increased with increasing villus height on the 7th POD. Enteral supplementation with alanyl-glutamine but not glutamine + alanine mixture promotes intestinal adaptation as evidenced by increased peptide transport after intestinal resection.
尽管父母给予谷氨酰胺可促进肠道适应性，但小肠切除术后肠内给予谷氨酰胺是否有效仍存在争议。为了进一步评估肠内补充的益处，必须考虑肽和氨基酸肽转运体的功能。我们基于大量小肠切除术后肽和氨基酸转运体表达的改变，评估了肠内丙氨酰 - 谷氨酰胺的效果。大鼠接受了80%的近端小肠切除术。最初通过Northern印迹分析检测残留肠细胞中谷氨酰胺酶（GA）、氨基酸转运体B0和肽转运体PepT1 mRNA的表达。小肠切除的大鼠从术前一天开始接受谷氨酰胺（2.0 g/kg/天）+丙氨酸（1.22 g/kg/天）混合物、丙氨酰 - 谷氨酰胺（2.972/kg/天）或生理盐水的大剂量补充，持续3天。在术后第3天（POD）和第7天POD，切除残留肠组织，并测量黏膜参数。GA活性和GA mRNA在第1天POD显著增加。尽管B0 mRNA水平逐渐下降，但PepT1 mRNA在术后增加，并在第5天POD达到初始水平的150%。在给予丙氨酰 - 谷氨酰胺的大鼠中，第7天POD时黏膜湿重和蛋白质含量随绒毛高度增加而同样增加。肠内补充丙氨酰 - 谷氨酰胺而非谷氨酰胺+丙氨酸混合物可促进肠道适应性，这在肠切除术后肽转运增加中得到证实。
