评估一种含溴化十六烷基三甲铵、卡那霉素和萘啶酸的新型琼脂培养基用于临床样本中铜绿假单胞菌的分离及色素生成增强。
Evaluation of a new agar medium containing cetrimide, kanamycin and nalidixic acid for isolation and enhancement of pigment production of Pseudomonas aeruginosa in clinical samples.
作者信息
Kodaka Hidemasa, Iwata Morihiro, Yumoto Shigeo, Kashitani Fusako
机构信息
Department of Microbiology, School of Medicine, Toho University, 5-21-16, Omori-nishi, Otaku, Tokyo 143-8540, Japan.
出版信息
J Basic Microbiol. 2003;43(5):407-13. doi: 10.1002/jobm.200310264.
A new selective agar medium (CKNA), containing cetrimide 0.3 g/l, kanamycin sulfate 50 mg/l, and nalidixic acid 5 mg/l, was developed for the isolation of Pseudomonas aeruginosa. It was compared to Nalidixic Acid Cetrimide agar (NAC), Pseudomonas Aeruginosa Selective Agar (PASA) and Pseudosel(TM) agar (CET) using 1,148 clinical specimens. The sensitivities rates of P. aeruginosa with CKNA, NAC, PASA, and CET were 88.2%, 81.3%, 79.2%, and 84.0%, respectively. The specificities of CKNA, NAC, PASA, and CET were 99.2%, 98.4%, 99.2%, and 99.7%, respectively.
一种新的选择性琼脂培养基（CKNA）被开发用于分离铜绿假单胞菌，该培养基含有0.3 g/l的溴化十六烷基三甲铵、50 mg/l的硫酸卡那霉素和5 mg/l的萘啶酸。使用1148份临床标本将其与萘啶酸十六烷基三甲基溴化铵琼脂（NAC）、铜绿假单胞菌选择性琼脂（PASA）和假单胞菌选择琼脂（CET）进行比较。CKNA、NAC、PASA和CET对铜绿假单胞菌的敏感性分别为88.2%、81.3%、79.2%和84.0%。CKNA、NAC、PASA和CET的特异性分别为99.2%、98.4%、99.2%和99.7%。
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