Hinde Paul, Meadows Jane, Saunders Jon, Edwards Clive
School of Biological Sciences, University of Liverpool, Liverpool, L69 7ZB United Kingdom.
Adv Appl Microbiol. 2003;52:29-74. doi: 10.1016/s0065-2164(03)01002-5.
DNA recombinases show some promise as reporters of pollutants providing that appropriate promoters are used and that the apparent dependence of expression on cell density can be solved. Further work is in progress using different recombinases and other promoters to optimize recombinase expression as well as to test these genetic constructs in contaminated environmental samples such as soil and water. It may be that a graded response reflecting pollutant concentration may not be possible. However, they show great promise for providing definitive detection systems for the presence of a pollutant and may be applicable to address the problem of bioavailability of pollutants in complex environments such as soil.
如果使用合适的启动子并且能够解决表达对细胞密度的明显依赖性问题，DNA重组酶作为污染物的报告分子显示出一定的前景。目前正在开展进一步的工作，使用不同的重组酶和其他启动子来优化重组酶的表达，并在土壤和水等受污染的环境样品中测试这些基因构建体。可能无法实现反映污染物浓度的分级响应。然而，它们在提供污染物存在的确切检测系统方面显示出巨大的前景，并且可能适用于解决复杂环境（如土壤）中污染物的生物可利用性问题。
