He Wei, Yuan Sheng, Zhong Wen-Hui, Siddikee Md Ashaduzzaman, Dai Chuan-Chao
College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.
College of Geography Sciences, Nanjing Normal University, Nanjing, 210023, China.
Appl Microbiol Biotechnol. 2016 Feb;100(3):1109-1119. doi: 10.1007/s00253-015-7160-6. Epub 2015 Nov 28.
The progress of genetically engineered microbial whole-cell biosensors for chemosensing and monitoring has been developed in the last 20 years. Those biosensors respond to target chemicals and produce output signals, which offer a simple and alternative way of assessment approaches. As actual pollution caused by human activities usually contains a combination of different chemical substances, how to employ those biosensors to accurately detect real contaminant samples and evaluate biological effects of the combined chemicals has become a realistic object of environmental researches. In this review, we outlined different types of the recent method of genetically engineered microbial whole-cell biosensors for combined chemical evaluation, epitomized their detection performance, threshold, specificity, and application progress that have been achieved up to now. We also discussed the applicability and limitations of this biosensor technology and analyzed the optimum conditions for their environmental assessment in a combined way.
在过去20年里，用于化学传感和监测的基因工程微生物全细胞生物传感器取得了进展。这些生物传感器对目标化学物质做出反应并产生输出信号，提供了一种简单且可供选择的评估方法。由于人类活动造成的实际污染通常包含多种化学物质的组合，如何利用这些生物传感器准确检测实际污染物样本并评估混合化学物质的生物效应，已成为环境研究的一个现实目标。在本综述中，我们概述了用于混合化学评估的基因工程微生物全细胞生物传感器的不同类型的最新方法，概括了它们目前已实现的检测性能、阈值、特异性和应用进展。我们还讨论了这种生物传感器技术的适用性和局限性，并综合分析了其用于环境评估的最佳条件。
