Ron Eliora Z
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Tel Aviv 69978, Israel.
Curr Opin Biotechnol. 2007 Jun;18(3):252-6. doi: 10.1016/j.copbio.2007.05.005. Epub 2007 May 25.
Whole-cell biosensors are finding increasing use for the detection of environmental pollution and toxicity. These biosensors are constructed through the fusion of promoters, responsive to the relevant environmental conditions, to easily monitored reporter genes. Depending on the choice of reporter gene, expression can be monitored by the production of colour, light, fluorescence or electrochemical reactions. Recent advances in this area have included the development of biosensors of compact size that enable the on-line and in situ monitoring of a large number of environmental parameters.
全细胞生物传感器在环境污染和毒性检测中的应用越来越广泛。这些生物传感器是通过将对相关环境条件有响应的启动子与易于监测的报告基因融合构建而成。根据报告基因的选择,可以通过颜色、光、荧光或电化学反应的产生来监测表达情况。该领域的最新进展包括开发出尺寸紧凑的生物传感器,能够对大量环境参数进行在线和原位监测。