Ono M A, Kishima M O, Itano E N, Bracarense A P F R L, Camargo Z P
Universidade Estadual de Londrina, Departamento de Ciências Patológicas, Londrina, PR, Brazil.
Med Mycol. 2003 Jun;41(3):265-8. doi: 10.1080/13693780310001597395.
The aim of this study was to evaluate the susceptibility of dogs to develop paracoccidioidomycosis by experimental infection. Puppies were inoculated with Paracoccidioides brasiliensis by an intravenous route and two out of four died 1 week postinoculation, showing, at histopathological analysis, granulomas in the lungs, spleen and liver. P. brasiliensis was isolated from these organs. The animals that survived the infection showed a strong reaction when skin was tested with gp43, a specific antigen of P. brasiliensis. These animals were killed at 1 and 5 months after infection, and no lesions, macroscopic or microscopic, were observed in the lungs, spleen or liver; furthermore no P. brasiliensis culture was obtained from these organs. These results suggest that dogs can develop paracoccidioidomycosis and reinforces the importance of this animal as a sensitive indicator of P. brasiliensis in the environment.
本研究的目的是通过实验性感染评估犬类感染副球孢子菌病的易感性。幼犬通过静脉途径接种巴西副球孢子菌，接种后1周，4只中有2只死亡，组织病理学分析显示肺、脾和肝中有肉芽肿。从这些器官中分离出了巴西副球孢子菌。在感染中存活下来的动物在用巴西副球孢子菌的特异性抗原gp43进行皮肤试验时表现出强烈反应。这些动物在感染后1个月和5个月时被处死，在肺、脾或肝中未观察到宏观或微观病变；此外，从这些器官中未获得巴西副球孢子菌培养物。这些结果表明犬类可感染副球孢子菌病，并强化了这种动物作为环境中巴西副球孢子菌敏感指示物的重要性。
