Khan Wajahatullah, Prithiviraj Balakrishnan, Smith Donald L
Department of Plant Science, Macdonald Campus of McGill University, 21,111 Lakeshore, Ste-Anne-de-Bellevue, QC, H9X 3V9, Canada.
J Plant Physiol. 2003 Aug;160(8):859-63. doi: 10.1078/0176-1617-00905.
Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) and tyrosine ammonia-lyase (TAL, 4.3.1.), the key enzymes of the phenylpropanoid pathway, are inducible in response to biotic (such as chitin from fungal cell walls) and abiotic cues. Application of chitin and chitosan to soybean leaf tissues caused increased activity of PAL and TAL enzymes. The elevation of enzyme activity was dependent on the chain length of the oligomers and time after treatment. The hexamer of chitin and pentamer of chitosan produced the maximum activities at 36 h after treatment as compared to controls. Total phenolic content of soybean leaves increased following chitosan and chitin oligomer treatments, showing a positive correlation between enzyme activity and total phenolic content.
苯丙氨酸解氨酶（PAL，EC 4.3.1.5）和酪氨酸解氨酶（TAL，4.3.1.）是苯丙烷类途径的关键酶，可响应生物（如来自真菌细胞壁的几丁质）和非生物信号而被诱导。将几丁质和壳聚糖应用于大豆叶片组织会导致PAL和TAL酶的活性增加。酶活性的升高取决于寡聚物的链长和处理后的时间。与对照相比，几丁质六聚体和壳聚糖五聚体在处理后36小时产生最大活性。壳聚糖和几丁质寡聚物处理后，大豆叶片的总酚含量增加，表明酶活性与总酚含量之间呈正相关。
