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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Eur J Biochem. 1977 Feb 15;73(1):255-60. doi: 10.1111/j.1432-1033.1977.tb11314.x.

Antibody toward phenylalanine ammonia-lyase of root tissue of sweet potato injured by cutting was obtained by immunizing of a rabbit with purified enzyme. 2. Phenylalanine ammonia-lyase activity developed markedly in disks of sweet potato roots in response to cut injury, reached a maximum after 12 h, and then decreased thereafter. Phenylalanine ammonia-lyase content, which was determined by the quantitative immunoprecipitation method, changed in parallel with enzyme activity. The rate of incorporation of the label from [14C]leucine into phenylalanine ammonia-lyase initially increased with the time of incubation after slicing, reached a maximum in 6h of incubation, then remained at an almost constant level regardless of the decrease in enzyme activity. The results indicate that the increase in enzyme activity is due to de novo synthesis of phenylalanine ammonia-lyase and the subsequent decrease in activity is not based on decreased synthesis of phenylalanine ammonia-lyase.

用纯化的酶免疫兔子，获得了针对因切割而受伤的甘薯根组织中苯丙氨酸解氨酶的抗体。2. 甘薯根圆盘在切割损伤后苯丙氨酸解氨酶活性显著增强，12小时后达到最大值，此后下降。通过定量免疫沉淀法测定的苯丙氨酸解氨酶含量与酶活性平行变化。切片后，[14C]亮氨酸标记掺入苯丙氨酸解氨酶的速率最初随孵育时间增加，在孵育6小时时达到最大值，然后尽管酶活性下降，但仍保持在几乎恒定的水平。结果表明，酶活性的增加是由于苯丙氨酸解氨酶的从头合成，而随后活性的下降并非基于苯丙氨酸解氨酶合成的减少。

Eur J Biochem. 1977 Feb 15;73(1):255-60. doi: 10.1111/j.1432-1033.1977.tb11314.x.

Antibody toward phenylalanine ammonia-lyase of root tissue of sweet potato injured by cutting was obtained by immunizing of a rabbit with purified enzyme. 2. Phenylalanine ammonia-lyase activity developed markedly in disks of sweet potato roots in response to cut injury, reached a maximum after 12 h, and then decreased thereafter. Phenylalanine ammonia-lyase content, which was determined by the quantitative immunoprecipitation method, changed in parallel with enzyme activity. The rate of incorporation of the label from [14C]leucine into phenylalanine ammonia-lyase initially increased with the time of incubation after slicing, reached a maximum in 6h of incubation, then remained at an almost constant level regardless of the decrease in enzyme activity. The results indicate that the increase in enzyme activity is due to de novo synthesis of phenylalanine ammonia-lyase and the subsequent decrease in activity is not based on decreased synthesis of phenylalanine ammonia-lyase.

用纯化的酶免疫兔子，获得了针对因切割而受伤的甘薯根组织中苯丙氨酸解氨酶的抗体。2. 甘薯根圆盘在切割损伤后苯丙氨酸解氨酶活性显著增强，12小时后达到最大值，此后下降。通过定量免疫沉淀法测定的苯丙氨酸解氨酶含量与酶活性平行变化。切片后，[14C]亮氨酸标记掺入苯丙氨酸解氨酶的速率最初随孵育时间增加，在孵育6小时时达到最大值，然后尽管酶活性下降，但仍保持在几乎恒定的水平。结果表明，酶活性的增加是由于苯丙氨酸解氨酶的从头合成，而随后活性的下降并非基于苯丙氨酸解氨酶合成的减少。