Lacoux Jérôme, Duval Isabelle, Dupré Patricia, Gutierrez Laurent, Lesueur Sophie, Roger David, Lainé Eric
Laboratoire de Biotechnologies et Physiologie Végétales, Université de Picardie Jules Verne, Faculté des Sciences, 33, rue Saint-Leu, 80039 Amiens Cedex, France.
J Plant Physiol. 2003 Aug;160(8):977-9. doi: 10.1078/0176-1617-00786.
The regulatory region of the flax Lupme3 gene, which codes for a pectin methylesterase, contains two sequences (PB box) that are putative cis-active sequence elements thought to regulate transcription in pollen. The Lupme3 promoter was fused to the beta-glucuronidase (gus) reporter gene. The chimeric gene fusion was introduced into tobacco via Agrobacterium-mediated transformation. Expression of the reporter gene was monitored using a histochemical X-Gluc assay at different stages of pollen maturation and germination. The Lupme3 promoter was found to be active in germination-competent mature pollen and in pollen tube.
亚麻Lupme3基因的调控区域编码一种果胶甲酯酶，该区域包含两个序列（PB框），它们是被认为在花粉中调控转录的假定顺式作用序列元件。Lupme3启动子与β-葡萄糖醛酸酶（gus）报告基因融合。通过农杆菌介导的转化将嵌合基因融合体导入烟草。使用组织化学X-Gluc测定法在花粉成熟和萌发的不同阶段监测报告基因的表达。发现Lupme3启动子在具有萌发能力的成熟花粉和花粉管中具有活性。
