Lacoux J, Gutierrez L, Dantin F, Beaudoin B, Roger D, Lainé E
Laboratoire de Biotechnologies et Physiologie Végétales, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue Saint-Luek, 80039 Amiens, France.
Protoplasma. 2003;222(3-4):205-9. doi: 10.1007/s00709-003-0019-1. Epub 2003 Oct 29.
Antisense transgenesis of tobacco (Nicotiana tabacum) with a partial flax (Linum usitatissimum L.) pectin methylesterase (Lupme3) cDNA sequence yielded plants with altered pollen content. Moreover, the characteristically sculptured cell wall surrounding the pollen grains was modified in transgenic tobacco plants: the wavy ornamentation was dramatically reduced, suggesting the involvement of the demethylation of pectin in the pollen cell wall-specific structure. Germination of pollen was decreased and the pollen tube surface aspect was also different in transgenic plants.
用部分亚麻（亚麻属）果胶甲酯酶（Lupme3）cDNA序列对烟草（烟草属）进行反义转基因，得到了花粉含量改变的植株。此外，转基因烟草植株中围绕花粉粒的具有特征性雕刻的细胞壁发生了改变：波浪状纹饰显著减少，这表明果胶去甲基化参与了花粉细胞壁特异性结构的形成。转基因植株中花粉萌发率降低，花粉管表面形态也有所不同。
