Picková Andrea, Paul Jan, Petruzzella Vittoria, Houstek Josef
Institute of Physiology and Centre for Integrated Genomics, Academy of Sciences of the Czech Republic, 142 20 4 - Krc, Prague, Czech Republic.
FEBS Lett. 2003 Sep 11;551(1-3):42-6. doi: 10.1016/s0014-5793(03)00890-1.
Atp11p (Atpaf1p; F(1)-ATPase assembly factor 1) and Atp12p (Atpaf2p; F(1)-ATPase assembly factor 2) are proteins required for the assembly of beta (F(1)-beta) and alpha (F(1)-alpha) subunits into the mitochondrial ATPase. Here we report about 100 times lower levels of ATPAF1 and ATPAF2 transcripts in relation to the mRNA levels of F(1)-alpha and F(1)-beta in a range of mouse tissues. Quantitative reverse-transcription polymerase chain reaction revealed nearly constant ATPAF1 expression in all tissues in both adult and 5-day-old mice (up to two-fold differences), indicating that ATPAF1 rather behaves like a maintenance gene. In contrast, ATPAF2 expression differed up to 30-fold in the tissues analysed. ATPAF2 tissue-specific expression was also found to correlate well with mRNA levels of both F(1)-alpha and F(1)-beta (BATz.Gt;kidney, liver>heart, brain>skeletal muscle), showing the highest mRNA level in the thermogenic, ATPase-poor brown adipose tissue, which is characterised by a 10-fold decrease in ATPase/respiratory chain stoichiometry relative to the other tissues.
Atp11p（Atpaf1p；F₁ - ATP酶组装因子1）和Atp12p（Atpaf2p；F₁ - ATP酶组装因子2）是β（F₁ - β）和α（F₁ - α）亚基组装到线粒体ATP酶中所必需的蛋白质。在此我们报告，在一系列小鼠组织中，与F₁ - α和F₁ - β的mRNA水平相比，ATPAF1和ATPAF2转录本水平低约100倍。定量逆转录聚合酶链反应显示，在成年和5日龄小鼠的所有组织中，ATPAF1表达几乎恒定（差异高达两倍），这表明ATPAF1更像是一个维持基因。相比之下，在分析的组织中，ATPAF2表达差异高达30倍。还发现ATPAF2的组织特异性表达与F₁ - α和F₁ - β的mRNA水平密切相关（棕色脂肪组织＞肾脏、肝脏＞心脏、大脑＞骨骼肌），在产热的、ATP酶含量低的棕色脂肪组织中mRNA水平最高，该组织的特点是相对于其他组织，ATP酶/呼吸链化学计量比降低了10倍。
