Houstĕk J, Tvrdík P, Pavelka S, Baudysová M
Institute of Physiology, Czechoslovak Academy of Sciences, Prague.
FEBS Lett. 1991 Dec 9;294(3):191-4. doi: 10.1016/0014-5793(91)80666-q.
The mRNA levels of ATPase beta, ATPase 6, cytochrome oxidase (COX) VIb and COX I subunits were found to be 2.4-13.8-fold higher in brown adipose tissue (BAT) than in heart, skeletal muscle, brain and liver of mice. The comparison with tissue contents of ATPase and COX revealed that the selective, 5-11-fold reduction of ATPase in BAT is not caused by decreased transcription of ATPase genes. Likewise, the ATPase beta and COX VIb mRNA levels in cultured brown adipocytes were also not influenced by norepinephrine, which activated the expression of the UCP gene by two orders of magnitude. The results indicate that the biosynthesis of mitochondrial ATPase in BAT is post-transcriptionally regulated.
研究发现，在小鼠的棕色脂肪组织（BAT）中，ATP酶β、ATP酶6、细胞色素氧化酶（COX）VIb和COX I亚基的mRNA水平比心脏、骨骼肌、大脑和肝脏中的高2.4至13.8倍。与ATP酶和COX的组织含量进行比较后发现，BAT中ATP酶选择性降低5至11倍并非由ATP酶基因转录减少所致。同样，培养的棕色脂肪细胞中ATP酶β和COX VIb的mRNA水平也不受去甲肾上腺素的影响，而去甲肾上腺素能使UCP基因的表达提高两个数量级。结果表明，BAT中线粒体ATP酶的生物合成受到转录后调控。
