使用组织工程化肺动脉进行跨物种猪内源性逆转录病毒传播的体内模型
In vivo model for cross-species porcine endogenous retrovirus transmission using tissue engineered pulmonary arteries.
作者信息
Walles Thorsten, Lichtenberg Arthur, Puschmann Carmen, Leyh Rainer, Wilhelmi Mathias, Kallenbach Klaus, Haverich Axel, Mertsching Heike
机构信息
Department of Cardiothoracic and Vascular Surgery, Medical School Hannover, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany.
出版信息
Eur J Cardiothorac Surg. 2003 Sep;24(3):358-63. doi: 10.1016/s1010-7940(03)00334-8.
OBJECTIVE
Acellularised porcine scaffolds have been successfully used for cardiovascular tissue engineering. However, there is concern about the possibility of porcine endogenous retrovirus (PERV) transmission. In this study we developed an in vivo model for cross-species PERV transmission.
METHODS
In vitro autologous repopulated porcine pulmonary arteries (n=6) were implanted in sheep in orthotopic position. Blood samples were collected regularly up to 6 months after implantation and tested for PERV by means of polymerase chain reaction and reverse transcriptase-polymerase chain reaction. Explanted tissue engineered pulmonary arteries were tested for PERV sequences.
RESULTS
PERV DNA was detectable in acellularised porcine scaffolds. No PERV sequences were detectable 6 months after implantation of in vitro repopulated acellularised porcine pulmonary arteries and in all tested peripheral blood samples.
CONCLUSIONS
Acellularised porcine matrix scaffolds can be used for cardiovascular tissue engineering of autologous grafts without risk of PERV transmission.
目的
去细胞猪支架已成功用于心血管组织工程。然而，人们担心猪内源性逆转录病毒（PERV）传播的可能性。在本研究中，我们建立了一种用于跨物种PERV传播的体内模型。
方法
将体外自体再植入的猪肺动脉（n = 6）原位植入绵羊体内。在植入后长达6个月的时间内定期采集血样，并通过聚合酶链反应和逆转录酶 - 聚合酶链反应检测PERV。对取出的组织工程化肺动脉进行PERV序列检测。
结果
在去细胞猪支架中可检测到PERV DNA。在体外再植入的去细胞猪肺动脉植入6个月后以及所有测试的外周血样本中均未检测到PERV序列。
结论
去细胞猪基质支架可用于自体移植物的心血管组织工程，而无PERV传播风险。
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