基于端粒序列的双重PCR检测法检测克氏锥虫和兰氏锥虫感染
Detection of Trypanosoma cruzi and Trypanosoma rangeli infection by duplex PCR assay based on telomeric sequences.
作者信息
Chiurillo Miguel Angel, Crisante Gladys, Rojas Agustina, Peralta Andreina, Dias Manuel, Guevara Palmira, Añez Néstor, Ramírez José Luis
机构信息
Decanato de Medicina, Universidad Centroccidental Lisandro Alvarado, Barquisimeto 3001, Venezuela.
出版信息
Clin Diagn Lab Immunol. 2003 Sep;10(5):775-9. doi: 10.1128/cdli.10.5.775-779.2003.
Abstract
We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to generate a duplex PCR assay for the simultaneous detection of Trypanosoma cruzi and Trypanosoma rangeli in experimentally and naturally infected triatomine (Reduviid) bugs and in infected human subjects. The assay was species specific and was capable of detecting 1/20th of T. cruzi and 1/4th of T. rangeli cell equivalents without complementary hybridization. In addition, the PCR-based assay was robust enough for direct application to difficult biological samples such as Reduviid feces or guts and was capable of recognizing all T. cruzi and T. rangeli strains and lineages. Because the assay primers amplify entirely different target sequences, no reaction interference was observed, facilitating future adaptation of this assay to an automated format.
摘要
我们利用端粒下动基体序列的物种特异性和重复性，开发了一种双重PCR检测方法，用于同时检测实验感染和自然感染的锥蝽（猎蝽科）臭虫以及感染人类受试者体内的克氏锥虫和兰氏锥虫。该检测方法具有物种特异性，无需互补杂交即可检测到1/20的克氏锥虫细胞当量和1/4的兰氏锥虫细胞当量。此外，基于PCR的检测方法足够稳健，可直接应用于如猎蝽粪便或肠道等难以处理的生物样本，并且能够识别所有克氏锥虫和兰氏锥虫菌株及谱系。由于检测引物扩增的是完全不同的靶序列，未观察到反应干扰，这便于该检测方法未来采用自动化形式。
相似文献
1
Detection of Trypanosoma cruzi and Trypanosoma rangeli infection by duplex PCR assay based on telomeric sequences.
Clin Diagn Lab Immunol. 2003 Sep;10(5):775-9. doi: 10.1128/cdli.10.5.775-779.2003.
2
Species specific detection of Trypanosoma cruzi and Trypanosoma rangeli in vector and mammalian hosts by polymerase chain reaction amplification of kinetoplast minicircle DNA.
Acta Trop. 1999 Mar 15;72(2):203-12. doi: 10.1016/s0001-706x(98)00085-0.
3
Amplification of a specific repetitive DNA sequence for Trypanosoma rangeli identification and its potential application in epidemiological investigations.
Exp Parasitol. 2000 Nov;96(3):147-59. doi: 10.1006/expr.2000.4563.
4
Detection of Trypanosoma cruzi and Trypanosoma rangeli infection in triatomine vectors by amplification of the histone H2A/SIRE and the sno-RNA-C11 genes.
Rev Inst Med Trop Sao Paulo. 2007 Jan-Feb;49(1):23-30. doi: 10.1590/s0036-46652007000100005.
5
Differentiation between Trypanosoma cruzi and Trypanosoma rangeli using heat-shock protein 70 polymorphisms.
Trop Med Int Health. 2014 Feb;19(2):195-206. doi: 10.1111/tmi.12222. Epub 2013 Nov 13.
6
Species-specific markers for the differential diagnosis of Trypanosoma cruzi and Trypanosoma rangeli and polymorphisms detection in Trypanosoma rangeli.
Parasitol Res. 2014 Jun;113(6):2199-207. doi: 10.1007/s00436-014-3872-2. Epub 2014 Apr 12.
7
Characterization of a candidate Trypanosoma rangeli small nucleolar RNA gene and its application in a PCR-based parasite detection.
Exp Parasitol. 2002 Oct;102(2):72-80. doi: 10.1016/s0014-4894(03)00027-4.
8
A simplified method for sample collection and DNA isolation for polymerase chain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors.
Mem Inst Oswaldo Cruz. 2000 Nov-Dec;95(6):863-6. doi: 10.1590/s0074-02762000000600021.
9
Identification and lineage genotyping of South American trypanosomes using fluorescent fragment length barcoding.
Infect Genet Evol. 2011 Jan;11(1):44-51. doi: 10.1016/j.meegid.2010.10.012. Epub 2010 Oct 26.
10
[Review of the biologic and diagnostic aspects of Trypanosoma (Herpetosoma) rangeli].
Rev Soc Bras Med Trop. 1998 Mar-Apr;31(2):207-20. doi: 10.1590/s0037-86821998000200007.
引用本文的文献
1
Infections and Coinfections by Trypanosomatid Parasites in a Rural Community of Venezuela.
Acta Parasitol. 2022 Jun;67(2):1015-1023. doi: 10.1007/s11686-021-00505-1. Epub 2022 Jan 11.
2
Regional biogeography of microbiota composition in the Chagas disease vector Rhodnius pallescens.
Parasit Vectors. 2019 Oct 29;12(1):504. doi: 10.1186/s13071-019-3761-8.
3
Giardia Infection and Trypanosoma Cruzi Exposure in Dogs in the Bosawás Biosphere Reserve, Nicaragua.
Ecohealth. 2019 Sep;16(3):512-522. doi: 10.1007/s10393-019-01434-2. Epub 2019 Aug 14.
4
Limit of detection of PCR/RFLP analysis of cytochrome oxidase II for the identification of genetic groups of Trypanosoma cruzi and Trypanosoma rangeli in biological material from vertebrate hosts.
Parasitol Res. 2018 Aug;117(8):2403-2410. doi: 10.1007/s00436-018-5928-1. Epub 2018 Jun 1.
5
Detection and genotyping of Trypanosoma cruzi from açai products commercialized in Rio de Janeiro and Pará, Brazil.
Parasit Vectors. 2018 Apr 10;11(1):233. doi: 10.1186/s13071-018-2699-6.
6
DNA content analysis allows discrimination between Trypanosoma cruzi and Trypanosoma rangeli.
PLoS One. 2017 Dec 19;12(12):e0189907. doi: 10.1371/journal.pone.0189907. eCollection 2017.
7
Development of chemiluminescent lateral flow assay for the detection of nucleic acids.
Biosensors (Basel). 2012 Jan 18;2(1):32-42. doi: 10.3390/bios2010032.
8
Species-specific markers for the differential diagnosis of Trypanosoma cruzi and Trypanosoma rangeli and polymorphisms detection in Trypanosoma rangeli.
Parasitol Res. 2014 Jun;113(6):2199-207. doi: 10.1007/s00436-014-3872-2. Epub 2014 Apr 12.
9
Modulation of immune response in experimental Chagas disease.
World J Exp Med. 2013 Feb 20;3(1):1-10. doi: 10.5493/wjem.v3.i1.1.
10
Cytochrome oxidase subunit 2 gene allows simultaneous detection and typing of Trypanosoma rangeli and Trypanosoma cruzi.
Parasit Vectors. 2013 Dec 23;6:363. doi: 10.1186/1756-3305-6-363.
本文引用的文献
1
GROWTH AND DIFFERENTIATION IN TRYPANOSOMA CRUZI. I. ORIGIN OF METACYCLIC TRYPANOSOMES IN LIQUID MEDIA.
Rev Inst Med Trop Sao Paulo. 1964 May-Jun;6:93-100.
2
[MORPHOLOGICAL VARIATIONS OBSERVED IN DIFFERENT STRAINS OF TRYPANOSOMA CRUZI].
Rev Inst Med Trop Sao Paulo. 1963 Sep-Oct;5:220-4.
3
[Effect of cortisone in experimental Trypansoma cruzi infection; preliminary communication].
Bol Inf Parasit Chil. 1952 Apr-Jun;7(2):22-4.
4
An improved general approach for cloning and characterizing telomeres: the protozoan parasite Trypanosoma cruzi as model organism.
Gene. 2002 Jul 10;294(1-2):197-204. doi: 10.1016/s0378-1119(02)00768-0.
5
Comparative study of Trypanosoma rangeli and Trypanosoma cruzi telomeres.
Mol Biochem Parasitol. 2002 Apr 9;120(2):305-8. doi: 10.1016/s0166-6851(02)00005-1.
6
Detection of Leishmania causing visceral leishmaniasis in the Old and New Worlds by a polymerase chain reaction assay based on telomeric sequences.
Am J Trop Med Hyg. 2001 Nov;65(5):573-82. doi: 10.4269/ajtmh.2001.65.573.
7
Detection and significance of inapparent infection in Chagas disease in western Venezuela.
Am J Trop Med Hyg. 2001 Sep;65(3):227-32. doi: 10.4269/ajtmh.2001.65.227.
8
A mini-exon multiplex polymerase chain reaction to distinguish the major groups of Trypanosoma cruzi and T. rangeli in the Brazilian Amazon.
Trans R Soc Trop Med Hyg. 2001 Jan-Feb;95(1):97-9. doi: 10.1016/s0035-9203(01)90350-5.
9
Amplification of a specific repetitive DNA sequence for Trypanosoma rangeli identification and its potential application in epidemiological investigations.
Exp Parasitol. 2000 Nov;96(3):147-59. doi: 10.1006/expr.2000.4563.
10
Organization of telomeric and sub-telomeric regions of chromosomes from the protozoan parasite Trypanosoma cruzi.
Mol Biochem Parasitol. 1999 May 25;100(2):173-83. doi: 10.1016/s0166-6851(99)00047-x.
Zotero 插件