委内瑞拉农村社区中锥体虫寄生虫的感染和合并感染。
Infections and Coinfections by Trypanosomatid Parasites in a Rural Community of Venezuela.
机构信息
Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela.
Centro de Medicina Tropical de Oriente, Universidad de Oriente (UDO), Núcleo Anzoátegui, Barcelona, Estado Anzoátegui, Venezuela.
出版信息
Acta Parasitol. 2022 Jun;67(2):1015-1023. doi: 10.1007/s11686-021-00505-1. Epub 2022 Jan 11.
INTRODUCTION
Trypanosoma cruzi, Trypanosoma rangeli and Leishmania spp. are parasites that coexist in several endemic areas. The identification of these parasites in hosts is important for the control programs.
METHODS
216 samples from human blood (101), blood of other mammals (45) and triatomine intestinal content and hemolymph (70), from an endemic area of Venezuela, were analysed. The samples were evaluated by; serology (only humans) and PCR for T. cruzi in human, other mammals and triatomines, PCR for T. rangeli in mammals-including human and triatomines and PCR for Leishmania in mammals-including human.
RESULTS
The 9.9% of the human samples were positive for T. cruzi by serology, 11.9% by PCR, 4% for T. rangeli PCR and none for Leishmania spp. PCR. 60% of the samples of other mammals showed DNA amplification for T. cruzi, 42.2% for T. rangeli and 4.4% for Leishmania spp. 61.4% of the triatomine samples showed DNA amplification for T. cruzi and 10% for T. rangeli.
CONCLUSIONS
High T. cruzi infection was detected in mammals and triatomines compared with T. rangeli. Low leishmanial infection was detected in other mammals. It is the first time that T. cruzi/T. rangeli coinfection, in humans, Canis familiaris (dog), and Bos Taurus (cow), were reported world-wide, and that this coinfection was described in Tamandua tetradactyla (anteater) from Venezuela. The coinfection T. cruzi/T. rangeli in mammals-including humans and triatomines, and coinfection T. cruzi/Leishmania spp. in non-human mammals, show the risk for trypanosomic zoonoses in this endemic area.
简介
克氏锥虫、拉氏锥虫和利什曼原虫是几种地方病中共同存在的寄生虫。在宿主中鉴定这些寄生虫对于控制项目很重要。
方法
对来自委内瑞拉一个地方病疫区的 216 个人类血液（101 份）、其他哺乳动物血液（45 份）和 70 份三锥虫肠道内容物和血淋巴样本进行了分析。通过血清学（仅人类）和人类、其他哺乳动物和三锥虫中的 T. cruzi PCR、包括人类和三锥虫中的 T. rangeli PCR 以及包括人类在内的哺乳动物中的 Leishmania spp. PCR 评估了这些样本。
结果
9.9%的人类样本通过血清学检测呈 T. cruzi 阳性，11.9%通过 PCR 检测呈阳性，4%通过 T. rangeli PCR 检测呈阳性，无一例通过 Leishmania spp. PCR 检测呈阳性。60%的其他哺乳动物样本显示 T. cruzi 的 DNA 扩增，42.2%的 T. rangeli 和 4.4%的 Leishmania spp. 61.4%的三锥虫样本显示 T. cruzi 的 DNA 扩增，10%的 T. rangeli。
结论
与 T. rangeli 相比，在哺乳动物和三锥虫中检测到高 T. cruzi 感染。在其他哺乳动物中检测到低利什曼原虫感染。这是首次在全球范围内报告人类、犬（Canis familiaris）和牛（Bos Taurus）中 T. cruzi/T. rangeli 合并感染，也是首次在委内瑞拉的 Tamandua tetradactyla（食蚁兽）中描述这种合并感染。哺乳动物中包括人类和三锥虫的 T. cruzi/T. rangeli 合并感染，以及非人类哺乳动物中的 T. cruzi/Leishmania spp. 合并感染，表明该地方病疫区存在锥虫性人畜共患病的风险。
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