血清免疫球蛋白漏出液对小鼠肠道分泌物抗体免疫状态的贡献：不同采样程序的影响。
Contribution of serum immunoglobulin transudate to the antibody immune status of murine intestinal secretions: influence of different sampling procedures.
作者信息
Meckelein Barbara, Externest Dörthe, Schmidt M Alexander, Frey Andreas
机构信息
Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung, Westfälische Wilhelms-Universität, D-48149 Münster, Germany.
出版信息
Clin Diagn Lab Immunol. 2003 Sep;10(5):831-4. doi: 10.1128/cdli.10.5.831-834.2003.
Abstract
Serum immunoglobulin transudation into the murine gut after intragastric immunization with the model antigen ovalbumin and cholera toxin adjuvant was investigated with regard to the mucosal sampling technique applied. The levels of serum-derived immunoglobulin A (IgA) turned out to be lowest in feces, intermediate in gut lavage fluid specimens, and highest in filter wick-collected samples. However, these levels did not exceed 2% of total and specific IgA in any mucosal sample type, except after the administration of very high antigen doses (> or =1 mg of antigen per g of body weight), when transudation rates of up to 31% could be measured in filter wick-collected samples from individual animals. Luminal IgG was plasma transudate and/or bile borne and appeared to be reabsorbed at the mucosa to some extent.
摘要
研究了用模型抗原卵清蛋白和霍乱毒素佐剂进行胃内免疫后，血清免疫球蛋白渗入小鼠肠道的情况，并探讨了所应用的黏膜采样技术。血清来源的免疫球蛋白A（IgA）水平在粪便中最低，在肠灌洗液标本中处于中等水平，而在滤纸条收集的样本中最高。然而，除了给予非常高剂量的抗原（≥1毫克抗原/克体重）后，在任何黏膜样本类型中，这些水平都不超过总IgA和特异性IgA的2%，此时在个别动物的滤纸条收集样本中可测得高达31%的渗出率。腔内IgG是血浆渗出液和/或胆汁携带的，并且似乎在黏膜处有一定程度的重吸收。
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