Nedrud J G, Sigmund N
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Reg Immunol. 1990;3(5):217-22.
The effect of cholera toxin, a potent mucosal adjuvant, upon the murine immune response to both soybean meal, a regular dietary antigen, and ovalbumin, a simulated dietary antigen, was examined. Mice were orally immunized with a target antigen, Sendai virus, with and without cholera toxin as a mucosal adjuvant. Both serum and intestinal IgA and IgG anti-Sendai virus antibody responses were significantly increased when cholera toxin was used. For soybean meal, the major protein source in laboratory animal chows, however, no increases in serum antibody titers were found in animals which received oral cholera toxin plus virus. In addition, in mice orally immunized with ovalbumin plus cholera toxin (and virus), both serum and intestinal IgA and IgG antibody titers against ovalbumin were increased. Oral pre-immunization with ovalbumin prior to oral ovalbumin plus cholera toxin, however, resulted in no increase in either serum or intestinal anti-ovalbumin antibody responses. Thus, the use of cholera toxin as an oral adjuvant does not increase antibody responses against dietary antigens if there has been pre-exposure to the dietary antigen.
研究了强效黏膜佐剂霍乱毒素对小鼠针对常规饮食抗原大豆粕和模拟饮食抗原卵清蛋白的免疫反应的影响。小鼠口服接种目标抗原仙台病毒，同时使用或不使用霍乱毒素作为黏膜佐剂。使用霍乱毒素时，血清及肠道IgA和IgG抗仙台病毒抗体反应均显著增强。然而，对于实验动物饲料中的主要蛋白质来源大豆粕，接受口服霍乱毒素加病毒的动物血清抗体滴度未升高。此外，在口服卵清蛋白加霍乱毒素（及病毒）免疫的小鼠中，血清及肠道针对卵清蛋白的IgA和IgG抗体滴度均升高。然而，在口服卵清蛋白加霍乱毒素之前先口服卵清蛋白进行预免疫，血清或肠道抗卵清蛋白抗体反应均未增强。因此，如果事先接触过饮食抗原，使用霍乱毒素作为口服佐剂不会增强针对饮食抗原的抗体反应。
