Du Yangzhou, Fischer Tanya Z, Lee Lucille N, Lercher Lauren D, Dreyfus Cheryl F
Department of Neuroscience and Cell Biology, UMDNJ/Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
Dev Neurosci. 2003 Mar-Aug;25(2-4):116-26. doi: 10.1159/000072261.
To define the effects of neurotrophins on oligodendrocytes, we monitored NGF, BDNF and NT-3 actions on basal forebrain (BF) and cortical populations. NGF, BDNF and NT-3 applied to BF oligodendrocytes elicited increases in expression of myelin basic protein (MBP) and enhanced the numbers of MBP+ cells, without affecting total cell numbers. In the cortex, however, while NGF and NT-3 influenced MBP expression, BDNF was without effect. To explore this apparent regional difference in BDNF action, we compared expression of the neurotrophin receptors trkA, trkB and trkC. While BF cells expressed all three trks, cortical cells did not express the full-length BDNF receptor, trkB. Interestingly, in no case was any receptor expressed by all oligodendrocytes, indicating that oligodendrocytes may be heterogeneous within a brain region. The data suggest that BF oligodendrocytes are influenced by BDNF to express MBP and are distinct in this ability from cortical cells.
为了确定神经营养因子对少突胶质细胞的影响，我们监测了神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）对基底前脑（BF）和皮质细胞群的作用。将NGF、BDNF和NT-3应用于BF少突胶质细胞可引起髓鞘碱性蛋白（MBP）表达增加，并增加MBP+细胞的数量，而不影响细胞总数。然而，在皮质中，虽然NGF和NT-3影响MBP表达，但BDNF没有作用。为了探究BDNF作用的这种明显区域差异，我们比较了神经营养因子受体trkA、trkB和trkC的表达。虽然BF细胞表达所有三种trk，但皮质细胞不表达全长BDNF受体trkB。有趣的是，在任何情况下，并非所有少突胶质细胞都表达任何一种受体，这表明少突胶质细胞在脑区内可能是异质性的。数据表明，BF少突胶质细胞受BDNF影响表达MBP，并且在这种能力上与皮质细胞不同。
