Ahn Seung-Joon, Yoo Ji-Hyun, Lee Hyeon-Cheol, Kim Sang-Yong, Noh Bong-Soo, Kim Jung-Hoe, Lee Jung-Kul
BioNgene Co., Ltd. 10-1, 1Ka, Myungryun-Dong, Jongro-Ku, Seoul 110-521, Korea.
Biotechnol Lett. 2003 Jul;25(14):1179-83. doi: 10.1023/a:1024554728773.
Mutagenesis of Erwinia rhapontici was performed to enhance the production of isomaltulose from sucrose. A mutant strain, BN 68089, was obtained through a screening process involving automated and miniaturized cultivation in Bioscreen C. This high-throughput, miniaturized screening system was optimized to identify the mutant strain, which had a conversion yield (90%) and productivity (194 g l(-1) h(-1)). The BN 68089 mutant cells were immobilized in sodium alginate and when operated in a packed bed reactor gave a yield of 89% and a productivity of 144 g l(-1) h(-1) of at 30 degrees C, the optimal temperature. Immobilized BN 68089 cells exhibited 8% and 15% higher yield and productivity, respectively, than those of the wild-type strain.
对胡萝卜欧文氏菌进行诱变以提高从蔗糖生产异麦芽糖的产量。通过在Bioscreen C中进行自动化和小型化培养的筛选过程获得了突变菌株BN 68089。该高通量、小型化筛选系统经过优化以鉴定出具有转化率（90%）和生产力（194 g l⁻¹ h⁻¹）的突变菌株。将BN 68089突变细胞固定在海藻酸钠中，在填充床反应器中于30℃（最佳温度）运行时，产率为89%，生产力为144 g l⁻¹ h⁻¹。固定化的BN 68089细胞的产率和生产力分别比野生型菌株高8%和15%。
