Laboratory of Food Biochemistry, Department of Food Science, School of Food Engineering, University of Campinas-UNICAMP, Avenida Monteiro Lobato 80, CEP 13083-862, C.P.6121, Campinas, São Paulo, Brazil.
Appl Biochem Biotechnol. 2010 Sep;162(1):89-102. doi: 10.1007/s12010-009-8899-y. Epub 2010 Feb 5.
Isomaltulose was obtained from sucrose solution by immobilized cells of Erwinia sp. D12 using a batch and a continuous process. Parameters for sucrose conversion into isomaltulose were evaluated using both experimental design and response surface methodology. Erwinia sp. D12 cells were immobilized in different alginates, and the influence of substrate flow rate and concentration parameters to produce isomaltulose from sucrose were observed. Response surface methodology demonstrated that packed bed columns containing cells immobilized in low-viscosity sodium alginate (250 cP) presented a mean isomaltulose conversion rate of 47%. In a continuous process, both sucrose substrate concentration and substrate flow rate parameters had a significant effect (p < 0.05) and influenced the conversion of sucrose into isomaltulose. Higher conversion rates of sucrose into isomaltulose, from 53-75% were obtained using 75 g of immobilized cells at a substrate flow rate of 0.6 mL/min.
采用固定化细胞的分批和连续过程，从蔗糖溶液中获得异麦芽酮糖。使用实验设计和响应面法评估了将蔗糖转化为异麦芽酮糖的参数。将 D12 属欧文氏菌固定在不同的海藻酸盐中，并观察了底物流速和浓度参数对蔗糖生产异麦芽酮糖的影响。响应面法表明，含有低粘度海藻酸钠（250 cP）固定化细胞的填充床柱的异麦芽酮糖转化率平均值为 47%。在连续过程中，蔗糖底物浓度和底物流速参数均有显著影响（p < 0.05），并影响蔗糖转化为异麦芽酮糖。使用 75 g 固定化细胞，在 0.6 mL/min 的底物流速下，蔗糖转化为异麦芽酮糖的转化率高达 53-75%。
