通过原位产物去除增强半合成头孢菌素头孢克洛的酶促合成。
Enhanced enzymatic synthesis of a semi-synthetic cephalosprin, cefaclor, with in situ product removal.
作者信息
Yang Liu, Wei Dong-Zhi
机构信息
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, P.R. China.
出版信息
Biotechnol Lett. 2003 Jul;25(14):1195-8. doi: 10.1023/a:1024595823747.
In the enzymatic synthesis of cefaclor, 3-chloro-7-d-(2-phenylglycinamide)-3-cephem-4-carboxylic acid, from phenylglycine methyl ester and 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid, the in situ product could influence both the overall conversion and hydrolysis of the ester. Optimization of the parameters, such as pH 6.2, 5 degrees C and substrate molar ratio of 2:1, made in situ product removal improve the overall conversion from 64% to 85% (mol/mol).
在由苯甘氨酸甲酯和7-氨基去乙酰氧基甲基-3-氯头孢烷酸酶促合成头孢克洛（3-氯-7-d-（2-苯甘氨酰胺）-3-头孢烯-4-羧酸）的过程中，原位产物会影响酯的总转化率和水解。对诸如pH 6.2、5℃和底物摩尔比2:1等参数进行优化后，原位产物的去除使总转化率从64%提高到了85%（摩尔/摩尔）。
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