Sundholm Franciska, Tahvanainen Maria
Laboratory of Polymer Chemistry, University of Helsinki, P.O. Box 55, FIN-00014, Helsinki, Finland.
J Chromatogr A. 2003 Aug 8;1008(2):129-34. doi: 10.1016/s0021-9673(03)00974-9.
Hydrolytic degradation of cellulose was shown to take place during the activation procedure in distilled water during the dissolution procedure of cellulose samples from papers for size-exclusion chromatography analyses in the lithium chloride-N,N-dimethylacetamide (DMAc) solution system. The use of dilute aqueous sodium hydroxide solution in the activation procedure prevents hydrolytic degradation of cellulose during the dissolution procedure, especially in the case of samples of aged papers with low pH. The use of the freeze-drying technique provides samples of cellulose ready-made for dissolution in lithium chloride-N,N-dimethylacetamide solution.
在用于尺寸排阻色谱分析的纸张纤维素样品于氯化锂 - N，N - 二甲基乙酰胺（DMAc）溶液体系中溶解的过程中，纤维素的水解降解在蒸馏水中的活化步骤期间就已发生。在活化步骤中使用稀氢氧化钠水溶液可防止纤维素在溶解过程中发生水解降解，特别是对于低pH值的老化纸张样品而言。冷冻干燥技术的使用提供了可供直接溶解于氯化锂 - N，N - 二甲基乙酰胺溶液的纤维素样品。
