Du Qizhen, Jerz Gerold, Waibel Reiner, Winterhalter Peter
Institute of Food and Biological Engineering, Hangzhou University of Commerce, Hangzhou 310035, China.
J Chromatogr A. 2003 Aug 8;1008(2):173-80. doi: 10.1016/s0021-9673(03)00988-9.
The Chinese phytomedicinal formulation Sanqi Zongdai Pian, traditionally prepared from crude extracts from roots of Panax notoginseng (Araliaceae), contains highly polar dammarane saponins which were separated at a preparative scale using high-speed counter-current chromatography (HSCCC). In each operation, 283 mg methanolic extract of five tablets was separated and yielded pure 157, 17, 13 and 56 mg of ginsenoside-Rb1, notoginsenoside-R1, ginsenoside-Re and ginsenoside-Rg1, respectively, n-hexane-n-butanol-water (3:4:7, v/v/v) was used for the two-phase solvent system of the HSCCC separation. The chemical structures of three ginsenosides and one notoginsenoside were elaborated by means of electrospray ionization MS-MS and NMR analysis.
中国植物药制剂三七总甙片，传统上由五加科植物三七根的粗提取物制备而成，含有高极性达玛烷皂苷，采用高速逆流色谱法（HSCCC）进行制备规模的分离。每次操作中，对五片药的283毫克甲醇提取物进行分离，分别得到157、17、13和56毫克的人参皂苷-Rb1、三七皂苷-R1、人参皂苷-Re和人参皂苷-Rg1，正己烷-正丁醇-水（3:4:7，v/v/v）用于HSCCC分离的两相溶剂系统。通过电喷雾电离质谱-质谱联用和核磁共振分析对三种人参皂苷和一种三七皂苷进行了化学结构阐述。
