Ha Young Wan, Lim Soon Sung, Ha In Jin, Na Yun-Cheol, Seo Jung-Ju, Shin Heungsop, Son Sung Ho, Kim Yeong Shik
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 110-460, South Korea.
J Chromatogr A. 2007 Jun 1;1151(1-2):37-44. doi: 10.1016/j.chroma.2007.01.038. Epub 2007 Jan 13.
Ginseng (Panax ginseng C. A. Meyer) has been well known to have a variety of ginsenosides that show diverse biological activities. Especially, the components of ginsenosides are quite different depending on the processing method. Recently, there have been several reports showing that less polar ginsenosides from Korean red ginseng (steam-treated Panax ginseng) have potent biological activities such as radical scavenging, vasodilating and anti-tumor activities. In this study, we have isolated four known ginsenosides Rg3, Rk1, Rg5 and F4 from Korean red ginseng by high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD). The enriched saponin fraction (350 mg) was separated by using methylene chloride-methanol-water-isopropanol (6:6:4:1, v/v) as the two-phase solvent system and yielded 28.6 mg of Rg5, 26.6 mg of Rk1, 32.2 mg of Rg3 and 8.1 mg of F4. The purity of these ginsenosides was assessed by HPLC-ELSD to be over 95%, and their structures were characterized by electrospray ionization mass spectrometry (ESI-MS), (1)H NMR and (13)C NMR.
人参（Panax ginseng C. A. Meyer）因含有多种具有不同生物活性的人参皂苷而闻名。特别是，人参皂苷的成分会因加工方法的不同而有很大差异。最近，有几份报告表明，韩国红参（经蒸汽处理的人参）中极性较小的人参皂苷具有强大的生物活性，如自由基清除、血管舒张和抗肿瘤活性。在本研究中，我们通过高速逆流色谱法（HSCCC）结合蒸发光散射检测（ELSD）从韩国红参中分离出了四种已知的人参皂苷Rg3、Rk1、Rg5和F4。使用二氯甲烷 - 甲醇 - 水 - 异丙醇（6:6:4:1，v/v）作为两相溶剂系统，分离出350 mg的富集皂苷馏分，得到28.6 mg的Rg5、26.6 mg的Rk1、32.2 mg的Rg3和8.1 mg的F4。通过HPLC - ELSD评估这些人参皂苷的纯度超过95%，并通过电喷雾电离质谱（ESI - MS）、¹H NMR和¹³C NMR对其结构进行了表征。
