Tan Mui-Keng, Niessen Ludwig M
Elisabeth Macarthur Agricultural Institute, NSW Agriculture, PMB 8, Woodbridge Road, Menangle, NSW 2570, Australia.
Mycol Res. 2003 Jul;107(Pt 7):811-21. doi: 10.1017/s0953756203008013.
Genetic relationships of the complex of Fusarium species associated with crown rot and head blight in cereals and some species associated with plant diseases in general were examined by distance and maximum parsimony algorithms of their internal transcribed spacer sequences. The analysis clustered the complex of Fusarium species that causes root and crown rot and head blight of cereals and three other clusters of F. sambucinum, F. venenatum and F. poae into one clade. This group of Fustarium species was also found in this study to correspond to the group defined by the presence of the tri5 gene. The tri5 gene was recently reported to co-segregate with the locus governing the type of trichothecene produced, and probably maps in the trichothecene gene cluster. The other clusters of F. avenaceum, F. tricinctum, F. torulosum, F. oxysporum, F. verticillioides and F. solani did not have the tri5 gene. Although, F. pseudograminearum was phylogenetically close with the cluster of F. graminearum, F. culmorum and F. cerealis, it could be distinctly separated from them. The distinct genetic status of F. pseudograminearum from F. graminearum corroborated with other published molecular data and isozyme findings. The molecular analysis provided a simple diagnostic tool to differentiate fungi causing crown rot from those involved in head blight.
利用谷物中与冠腐病和赤霉病相关的镰刀菌复合体以及一些与一般植物病害相关的镰刀菌物种的内部转录间隔区序列，通过距离算法和最大简约算法研究了它们之间的遗传关系。分析将导致谷物根腐病、冠腐病和赤霉病的镰刀菌复合体以及其他三个簇，即接骨木镰刀菌、产毒镰刀菌和早熟禾镰刀菌，聚为一个进化枝。在本研究中还发现，这组镰刀菌物种与由tri5基因的存在所定义的组相对应。最近有报道称，tri5基因与控制单端孢霉烯产生类型的基因座共分离，并且可能位于单端孢霉烯基因簇中。燕麦镰刀菌、三线镰刀菌、串珠镰刀菌、尖孢镰刀菌、轮枝镰刀菌和茄病镰刀菌的其他簇没有tri5基因。虽然，伪禾谷镰刀菌在系统发育上与禾谷镰刀菌、燕麦镰刀菌和小麦镰刀菌簇接近，但它可以与它们明显区分开来。伪禾谷镰刀菌与禾谷镰刀菌不同的独特遗传地位与其他已发表的分子数据和同工酶研究结果一致。分子分析提供了一种简单的诊断工具，可区分引起冠腐病的真菌和引起赤霉病的真菌。
