Gilchrest Helen, Cheewatrakoolpong Boonlert, Billah Motasim, Egan Robert W, Anthes John C, Greenfeder Scott
Schering-Plough Research Institute, 2015 Galloping Hill Rd. MS-1600, Kenilworth, NJ 07033, USA.
Life Sci. 2003 Oct 3;73(20):2571-81. doi: 10.1016/s0024-3205(03)00607-6.
Mast cells are the central mediating cells of allergic reactions. Binding of allergen specific IgE to high affinity IgE receptor (Fcepsilon RI) and subsequent binding of allergen by the IgE causes receptor cross-linking and activation. In a study examining the differential gene expression in human cord blood-derived mast cells (CBMCs) mediated by activation of Fcepsilon RI both with IgE and IgE followed by cross-linking with alpha-IgE, the chemokine I-309 was found to be upregulated. I-309 is the ligand for the CCR8 receptor and is responsible for chemoattraction of TH2 type T-cells. Interestingly, I-309 RNA and protein levels were elevated not only in response to IgE/alpha-IgE activation but also by IgE alone. In addition, the I-309 levels were augmented by growth of the CBMCs in the presence of the proinflammatory cytokine IL-4. GM-CSF and MIP-1alpha secretion was also induced by IgE. These results suggest that IgE, through the production and release of cytokines such as I-309, GM-CSF and MIP-1alpha could promote an inflammatory reaction in the absence of antigen stimulation of mast cells.
肥大细胞是过敏反应的核心介导细胞。变应原特异性IgE与高亲和力IgE受体（Fcepsilon RI）结合，随后IgE与变应原结合导致受体交联和激活。在一项研究中，研究了用IgE以及IgE随后与α-IgE交联激活Fcepsilon RI介导的人脐血来源肥大细胞（CBMCs）中的差异基因表达，发现趋化因子I-309上调。I-309是CCR8受体的配体，负责TH2型T细胞的化学吸引。有趣的是，I-309 RNA和蛋白质水平不仅在对IgE/α-IgE激活的反应中升高，而且在单独使用IgE时也升高。此外，在促炎细胞因子IL-4存在的情况下，CBMCs的生长会增加I-309水平。IgE也诱导GM-CSF和MIP-1α分泌。这些结果表明，IgE通过产生和释放诸如I-309、GM-CSF和MIP-1α等细胞因子，在肥大细胞没有抗原刺激的情况下可能促进炎症反应。
