Gupta Atul, Rao Govind
Department of Chemical and Biochemical Engineering, University of Maryland-Baltimore County, 101 ECS Building, 1000 Hilltop Circle, Baltimore, MD 21250, USA.
Biotechnol Bioeng. 2003 Nov 5;84(3):351-8. doi: 10.1002/bit.10740.
We describe a study of oxygen transfer in shake flasks using a non-invasive optical sensor. This study investigates the effect of different plugs, presence of baffles, and the type of media on the dissolved oxygen profiles during Escherichia coli fermentation. We measured the volumetric mass transfer coefficient (k(L)a) under various conditions and also the resistances of the various plugs. Finally, we compared shake flask k(L)a with that from a stirred tank fermentor. By matching k(L)a's we were able to obtain similar growth and recombinant protein product formation kinetics in both a fermentor and a shake flask. These results provide a quantitative comparison of fermentations in a shake flask vs. a bench-scale fermentor and should be valuable in guiding scale-up efforts.
我们描述了一项使用非侵入式光学传感器对摇瓶中氧气传递进行的研究。本研究调查了不同瓶塞、挡板的存在以及培养基类型对大肠杆菌发酵过程中溶解氧分布的影响。我们测量了各种条件下的体积传质系数（k(L)a）以及各种瓶塞的阻力。最后，我们将摇瓶的k(L)a与搅拌罐发酵罐的k(L)a进行了比较。通过匹配k(L)a，我们能够在发酵罐和摇瓶中获得相似的生长和重组蛋白产物形成动力学。这些结果提供了摇瓶与实验室规模发酵罐中发酵的定量比较，在指导放大研究方面应具有重要价值。
