Yagci Nevin, Artan Nazik, Cokgör Emine Ubay, Randall Clifford W, Orhon Derin
Istanbul Technical University, ITU Insaat Fakultesi Cevre Muhendisligi Bolumu, Istanbul, Turkey.
Biotechnol Bioeng. 2003 Nov 5;84(3):359-73. doi: 10.1002/bit.10765.
This paper proposes a new metabolic model for acetate uptake by a mixed culture of phosphate- and glycogen-accumulating organisms (PAOs and GAOs) under anaerobic conditions. The model uses variable overall stoichiometry based on the assumption that PAOs may have the ability of using the glyoxylate pathway to produce the required reducing power for polyhydroxyalkonate (PHA) synthesis. The proposed model was tested and verified by experimental results. A sequencing batch reactor system was operated for enhanced biological phosphorus removal (EBPR) with acetate as the sole carbon source at different influent acetate/phosphate ratios. The resulting experimental data supported the validity of the proposed model, indicating the presence of GAOs for all tested HAc/P ratios, especially under P-limiting conditions. Strong agreement is observed between experimental values and model predictions for all model components, namely, PHB production, PHA composition, glycogen utilization, and P release.
本文提出了一种新的代谢模型，用于描述在厌氧条件下，聚磷菌和聚糖原菌（PAO和GAO）混合培养物对乙酸盐的摄取。该模型基于PAO可能具有利用乙醛酸途径产生聚羟基脂肪酸酯（PHA）合成所需还原力的能力这一假设，采用可变的总化学计量关系。所提出的模型通过实验结果进行了测试和验证。采用序批式反应器系统，以乙酸盐作为唯一碳源，在不同进水乙酸盐/磷酸盐比例下运行，用于强化生物除磷（EBPR）。所得实验数据支持了所提出模型的有效性，表明在所有测试的HAc/P比例下均存在GAO，特别是在磷限制条件下。对于所有模型组分，即聚β-羟基丁酸酯（PHB）产量、PHA组成、糖原利用和磷释放，实验值与模型预测值之间均观察到高度一致。
