Wong Chi-Huey, Bryan Marian C
Department of Chemistry, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92307, USA.
Methods Enzymol. 2003;362:218-25. doi: 10.1016/S0076-6879(03)01015-2.
Presented herein are a variety of methods for the attachment of saccharides to the surface of microtiter plates through long-chain hydrocarbons capable of binding to the polystyrene surface through noncovalent interaction. These glycolipids have the benefit of diverse and facile conjugation procedures and the ability to withstanding a range of biological assays. On addition of carbohydrates to the microtiter plate well, a suitable surface for both ELISA and enzymatic transformation is available, making the saccharide arrays useful for high-throughput study of specificity and inhibition in sugar-protein interactions.
本文介绍了多种通过长链烃将糖类连接到微量滴定板表面的方法，这些长链烃能够通过非共价相互作用与聚苯乙烯表面结合。这些糖脂具有多样且简便的共轭程序，以及能够经受一系列生物学检测的优点。在向微量滴定板孔中添加碳水化合物后，可获得适用于酶联免疫吸附测定（ELISA）和酶促转化的表面，这使得糖类阵列可用于高通量研究糖蛋白相互作用中的特异性和抑制作用。
