Haines D M, Martin K M, Chelack B J, Sargent R A, Outerbridge C A, Clark E G
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
J Vet Diagn Invest. 1999 Sep;11(5):396-9. doi: 10.1177/104063879901100502.
A reliable antemortem diagnostic method is needed for determining infection with canine distemper virus (CDV). The utility of immunohistochemical detection of CDV antigen was examined was examined for samples of nasal and footpad epithelium and haired skin in dogs with and without detectable CDV antigen in the lung and/or brain. Tissues from 57 dogs at risk of CDV infection were tested. Viral antigen was found in the lung and/or brain of 28 dogs. Among these dogs, viral antigen was demonstrated in the epithelial cells of the nasal mucosa in 24 of 27 dogs, in the footpad epithelium in 24 of 26 dogs, and in the haired skin of the dorsal neck in 26 of 27 dogs. Among the 29 dogs without CDV antigen in either the lung or brain, 1 dog had positive staining for viral antigen in the skin and nasal mucosa. Biopsies of haired skin of the dorsal neck, which is relatively simple to sample, can be used for immunohistochemical testing for acute and subacute infection with CDV.
需要一种可靠的生前诊断方法来确定犬瘟热病毒（CDV）感染情况。对于肺和/或脑中可检测到CDV抗原和未检测到CDV抗原的犬，研究了免疫组织化学检测CDV抗原在鼻和脚垫上皮以及有毛皮肤样本中的效用。对57只存在CDV感染风险的犬的组织进行了检测。在28只犬的肺和/或脑中发现了病毒抗原。在这些犬中，27只犬中有24只在鼻黏膜上皮细胞中检测到病毒抗原，26只犬中有24只在脚垫上皮中检测到病毒抗原，27只犬中有26只在颈背部有毛皮肤中检测到病毒抗原。在肺或脑中均无CDV抗原的29只犬中，有1只犬的皮肤和鼻黏膜病毒抗原染色呈阳性。颈背部有毛皮肤活检取样相对简单，可用于CDV急性和亚急性感染的免疫组织化学检测。
