Gröne A, Engelhardt P, Zurbriggen A
Länggassstr. 122, Postfach, 3001 Bern, Switzerland.
Vet Pathol. 2003 Sep;40(5):574-8. doi: 10.1354/vp.40-5-574.
The proliferation of footpad keratinocytes of canine distemper virus (CDV)-infected dogs was investigated. Footpads of 19 dogs inoculated experimentally with a virulent distemper strain (A75/17) and of two noninoculated control dogs were collected at necropsy. Dogs were divided into four groups according to results of the postmortem examination: dogs with severe distemper (group 1), dogs with mild distemper (group 2), inoculated dogs without distemper (group 3) and noninoculated dogs (group 4). There was no distinct difference of epidermal thickness among the four groups. Infection of the footpad epidermis with CDV was demonstrated using immunohistochemistry for viral nucleoprotein and in situ hybridization for nucleoprotein messenger ribonucleic acid (mRNA). Only group 1 dogs had viral antigen and mRNA in the footpad epidermis with the same distribution. Footpad epidermis of group 1 dogs had more mitotic figures in the basal layer, and significantly more basal keratinocytes were positive for the proliferation markers Ki-67 and proliferating cell nuclear antigen. Double-staining for Ki-67 and viral nucleoprotein identified rare double-labeled basal keratinocytes. These findings suggest that the presence of CDV particles in the footpad epidermis is associated with keratinocyte proliferation.
对犬瘟热病毒（CDV）感染犬脚垫角质形成细胞的增殖情况进行了研究。在尸检时收集了19只经强毒犬瘟热毒株（A75/17）实验接种的犬以及2只未接种的对照犬的脚垫。根据尸检结果将犬分为四组：患有严重犬瘟热的犬（第1组）、患有轻度犬瘟热的犬（第2组）、接种后未患犬瘟热的犬（第3组）和未接种的犬（第4组）。四组之间的表皮厚度没有明显差异。使用病毒核蛋白免疫组织化学和核蛋白信使核糖核酸（mRNA）原位杂交技术证实了脚垫表皮感染了CDV。只有第1组犬的脚垫表皮中有病毒抗原和mRNA，且分布相同。第1组犬的脚垫表皮基底层有更多的有丝分裂象，并且更多的基底角质形成细胞对增殖标志物Ki-67和增殖细胞核抗原呈阳性。Ki-67和病毒核蛋白的双重染色鉴定出罕见的双标记基底角质形成细胞。这些发现表明，脚垫表皮中CDV颗粒的存在与角质形成细胞增殖有关。
